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植物灰的基因转换系统的建立和AtPAP1的过度表达增强了干旱压力

Haimeng Zhang¹, Ruxin Wang¹, Hong Li¹

¹College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, Henan, 471023, China; Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang, Henan, 471023, China.

Plant physiology and biochemistry : PPB

|December 5, 2025

概括

这项研究建立了P. grisea的稳定基因转换系统,增强了其药用和装饰潜力. 在P. grisea中过度表达AtPAP1基因显著增加了干旱耐药性,通过增加素积累.

科学领域:

植物生物技术植物生物技术
基因工程是一种基因工程.
提高作物质量的方法.

背景情况:

灰植物 (P. grisea) 是一种具有药用和装饰价值的孤儿作物,缺乏成熟的基因转换系统.
开发这样一个系统对于理解基因功能至关重要,特别是在Solanaceae家族内的安托西亚尼合成和应激反应中.

研究的目的:

为了优化P. grisea.的再生系统.
为P. grisea.建立一个稳定的基因转换系统.
调查AtPAP1基因在增强转基因P. grisea. 素合成和抗干旱能力方面的功能.

主要方法:

优化再生协议使用 hypocotyls,cotyledons 和真叶作为扩展剂.
开发了一种Agrobacterium介导的转化系统,优化光密度,感染持续时间和共同培养时间.
产生过度表达AtPAP1基因的转基因P. grisea,并评估其对植物形态学,素积累和干旱耐受性的影响.

主要成果:

催化剂被证明是再生最有效的扩展剂,达到超过44.15%的诱导率.
通过使用优化参数,实现了稳定的转化系统,高峰转基因阳性率约为42.17%,使用优化参数.
在PAP1的过度表达显著增加了素积累,改变了植物形态,增强了抗干旱能力,由改善的生理参数 (POD,RWC) 和基因表达 (CHI,CHS) 证明.

结论:

一个强大的再生和稳定的Agrobacterium介导的P. grisea的转化系统已经成功建立.
AtPAP1基因的异质过度表达使P. grisea. 增强了对干旱的抵抗力.
这种转化系统为基因工程和在P. grisea.中开发抗干旱的生殖质提供了宝贵的工具.

关键词:

安托氨酸是一种安托氨酸.干旱造成的压力是干旱.基因转换系统是基因转换系统.灰色的植物 (Physalis grisea) 在

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