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在雄性Siniperca chuatsi中引起雌激素暴露的女性化:关于性别逆转,肝损伤和基因表达变化的多维研究
Kaichun Chen1, Weibin Li1, Weijian Chen1
1South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
概括
雌激素 (E1) 暴露使雄性Siniperca chuatsi鱼变得女性化,诱导丸卵巢转变并改变肝功能. 这种内分泌干扰会影响水生环境中的生殖健康和脂质代谢.
科学领域:
- 环境科学 环境科学
- 内分泌学 在内分泌学.
- 水生毒理学 水生毒理学
背景情况:
- 雌激素 (E1) 是水生环境中的天然雌激素污染物.
- E1可以破坏鱼类的生殖和内分泌系统.
研究的目的:
- 评估E1暴露对雄性Siniperca chuatsi的影响.
- 分析淋巴腺和肝脏组织学,酶活动和基因表达.
主要方法:
- 雄性S. chuatsi被暴露于E1 (0.0,0.01,0.1,1.0μg/L) 在60天内.
- 进行了组织学分析,酶活性测定 (SOD,T-AOC) 和转录基因分析.
主要成果:
- 高度的E1诱导了雄性鱼的80%的丸-卵巢转变.
- E1 导致肝损伤 (真空化,炎症) 和增加抗氧化酶活性.
- 转录组分析揭示了性别分化基因和脂质代谢途径的改变表达.
结论:
- 暴露于E1会导致氧化损伤,并改变男性S. chuatsi. 的类固醇激素合成和脂质代谢.
- 这些效应表明女性化内分泌干扰,导致雄性鱼的女性化.
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