分子相互作用和聚合乳清蛋白-Armillaria mellea多糖水凝的凝及其在酸奶中的应用
Xiaomeng Sun1, Ying Su2, Weibing Tao2
1Key Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China; Danisco (China) Co., LTD, Kunshan, Jiangsu, 215300, China.
International journal of biological macromolecules
|December 5, 2025
概括
聚合乳清蛋白 (PWP) 和Armillaria mellea多糖化合物 (AMP) 形成复合基,增强酸奶的质地和稳定性. 这种天然加厚剂改善了酸奶的质量.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物聚合物相互作用
- 材料科学 是一种材料科学.
背景情况:
- 乳清蛋白是一种常见的食品成分,具有功能性质.
- 自然的多糖类可以改变食物的质地和稳定性.
- 了解蛋白质-多糖相互作用是开发新型食品成分的关键.
研究的目的:
- 研究聚合乳清蛋白 (PWP) 和Armillaria mellea多糖 (AMP) 之间的相互作用机制.
- 评估PWP-AMP复合水凝作为酸奶中的天然加厚剂的应用.
- 描述水凝的结构性,热性,机械性和抗氧化性能.
主要方法:
- 同时的风学 - 里埃变换红外光谱学 (FTIR)
- 2D相关谱 (2D-COS) 是一种二维相关谱法.
- 分子对接模拟分子对接模拟
- 水凝特性 (热稳定性,机械强度,抗氧化能力) 的表征
- 酸奶的配方和感官评估.
主要成果:
- AMP通过结,疏水和静电相互作用增强了PWP凝网络.
- 复合水凝显示显著改善了热稳定性 (变质化温度增加了~17°C).
- 用6%的AMP-PWP水凝制成的酸奶表现出优越的质地,保持水分容量 (62.95%) 和感官特性.
- 抗氧化能力显著提高,DPPH和ABTS的激素清除率分别达到37.73%和75.40%.
结论:
- PWP-AMP复合水凝为酸奶提供了一个有前途的天然加厚和纹理系统.
- 这项研究为设计基于蛋白质多糖的水凝提供了理论基础.
- 这种方法表明了开发具有增强功能的新型天然食品成分的潜力.
更多相关视频
相关概念视频
Dehydration Synthesis
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.Synthesis of carbohydratesSugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from one reactant...
Hydrolysis
Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
Microorganisms in Agriculture and Food industry
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...


