相关实验视频

Updated: Jan 9, 2026

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

一个染色体层次的基因组组合的Macrobrachium hainanense

Chao Bian^1,2, Rujingwen Huan¹, Yiting Jin²

¹Laboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518057, China.

Scientific data

|December 5, 2025

概括

为经济上重要的Macrobrachium hainanense生成了一种高质量的基因组组. 这种基因组资源有助于了解其遗传基础,并支持保护和繁殖这一临灭绝的物种的努力.

科学领域:

水产养殖是水产养殖的一种方式.
基因组学就是基因组学.
海洋生物学海洋生物学

背景情况:

马克罗布拉希乌姆海南人 (Macrobrachium hainanense) 是一种无人类甲动物,是中国的关键经济物种.
由于过度捕捞和繁殖的挑战,M. hainanense的自然种群正在减少.

研究的目的:

为了生成M. hainanense. 的高质量的染色体水平基因组组合.
为保护,可持续发展和繁殖计划提供基因组资源.

主要方法:

组合PacBio HiFi测序和Hi-C技术用于基因组组装.
将组件固定在51个染色体上,识别了中间体和端粒.
用BUSCO.CO.进行注释的蛋白质编码基因和评估基因组完整性.

主要成果:

生成了一个4.3 Gb的染色体级基因组组件,具有87.0 Mb的支架N50.0.
成功将基因组固定在51个染色体上.
实现了93.8%的BUSCO完整性,并注释了46,945个蛋白质编码基因.

结论:

高质量的基因组组装是M. hainanense研究的宝贵资源.
这些基因组数据将促进未来的育种计划和杂交研究.
支持可持续利用和保护这一重要的经济实践.

更多相关视频

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

Published on: August 8, 2018

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Published on: June 15, 2017

相关概念视频

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.