单细胞转录组分析揭示了在LIHC开发过程中预后相关RNA结合蛋白的调控程序
En-di Zhang1, Chenxuan Li1, Zhuo Cheng1
1Organ Transplantation Center, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, Yunnan, 650032, China.
Cancer cell international
|December 5, 2025
概括
RNA结合蛋白 (RBPs) 在肝细胞癌 (HCC),一种肝癌中表现多样. 该研究确定HDGF是潜在的癌基因驱动HCC进展,并建议RBPs作为治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 肝细胞癌 (HCC) 是一种具有鲜为人知的RNA结合蛋白 (RBP) 表达异质性的侵袭性肝癌.
- 在单细胞水平上研究RBP异质性对于理解HCC进展和确定治疗点至关重要.
研究的目的:
- 为了研究HCC的单细胞水平RBP异质性.
- 探索RBPs在HCC进展中的监管作用.
- 评估RBPs在HCC中的治疗潜力.
主要方法:
- 单变Cox分析和NMF算法被用于选预后RBP和分层瘤集群.
- 单细胞RNA测序被用于分析恶性和髓质细胞衍生的细胞中的RBP异质性.
- 在体外测试 (CCK-8,EdU,Transwell) 和SpliceSeq分析验证了HDGF的作用.
主要成果:
- RBP表达与HCC等级有显著的相关性,预后RBP富含于核糖体生物发生路径.
- 单细胞分析揭示了原发性与复发性HCC的明显RBP模式,确定YRDC和HDGF作为关键调节者.
- HDGF促进了HCC细胞的增殖,迁移和入侵,并与生存相关的替代拼接事件有关.
结论:
- RBPs表现出单细胞异质性,并与HCC进展途径有关.
- 根据体外和拼接数据,HDGF被确定为瘤性RBP候选物.
- 包括HDGF在内的RBPs代表了未来HCC机制和治疗研究的有希望的目标.
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