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相关概念视频

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.5K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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DNA Topoisomerases02:02

DNA Topoisomerases

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Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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DNA Base Pairing02:27

DNA Base Pairing

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DNA Base Pairing02:27

DNA Base Pairing

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Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
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相关实验视频

Updated: Jan 9, 2026

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

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可逆序列依赖DNA凝聚与阿佐二间隔器.

Yunzhe Li1, Julie Pham1, Mathieu Morel1

  • 1CPCV, UMR8228, Department of Chemistry, PSL University, Sorbonne Université, CNRS, Ecole Normale Supérieure, 75005 Paris, France.

Langmuir : the ACS journal of surfaces and colloids
|December 6, 2025
PubMed
概括

研究人员使用一种新型DNA结合剂AzodiGua引入了对DNA协同体的序列敏感性. 这允许光控制的组装和拆卸,扩大生物材料和治疗学的应用.

科学领域:

  • 超分子化学 超分子化学
  • 生物材料科学 生物材料科学
  • 分子工程分子工程分子工程

背景情况:

  • 基因协同体是由静电相互作用和增量驱动的关联组件.
  • 目前的DNA同体缺乏序列特异性,限制了它们的应用.
  • 协动物中的高局部DNA度为治疗,仿生和生物感知提供了潜力.

研究的目的:

  • 将序列灵敏性引入DNA共聚.
  • 为了开发光敏DNA协同化物,使用基于阿佐的DNA结合剂.
  • 为了探索对DNA协同形成和溶解的可调节控制.

主要方法:

  • 使用一种基于亚博的DNA结合剂,AzodiGua,用于将DNA结合到基对中.
  • 研究了AzodiGua度对不同DNA结构 (双链 vs 单链) 和GC含量的协作用.
  • 在紫外线和蓝光照明下,通过 trans/cis 异构化对阿佐烯部分进行探索同类动物的光交换.
  • 研究了使用alpha-cyclodextrin来隔离跨-AzodiGua的逆光控制.

主要成果:

  • 阿佐迪瓜成功地传递了对DNA同的序列灵敏度.
  • 凝聚效率因DNA结构和GC基对分数/分布而有所不同.

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  • DNA协体表现出可逆光敏感性,在紫外线下溶解,在蓝光下重塑.
  • 通过alpha-cyclodextrin对trans-AzodiGua进行选择性封存,可以实现反向照相控制.

    • 结论:

    • AzodiGua 能够使特定序列和光敏感的 DNA 协同生长.
    • 通过光触发可实现对协体形成和溶解的控制.
    • 开发的系统为先进的生物材料和药物输送系统提供了新的策略.