广场脑干成像的协议在很大程度上保留了覆盖的小脑
Azumi Hatakeyama1, Chihiro Ito2, Daisuke Yamada3
1Institute for Quantum Life Science, National Institutes for Quantum Science and Technology (QST), Anagawa 4-9-1, Inage-ku, Chiba 263-8555, Japan; Faculty of Pharmaceutical Sciences, Tokyo University of Science, Niijuku 6-3-1, Katsushika-ku, Tokyo 125-8585, Japan.
STAR protocols
|December 6, 2025
概括
这项研究详细介绍了一种微侵袭的两光子成像协议,用于观察小鼠单独核通道 (NTS) 中的神经活动. 这种方法有助于理解NTS神经元在情绪和新陈代谢调节中的多种功能.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 生理学 生理学 生理学
背景情况:
- 单独导管核 (NTS) 是一个关键的大脑干区域,集成内脏感官信息.
- NTS神经元在调节情绪和新陈代谢方面发挥着关键作用.
- 了解NTS神经元异质性对于破译复杂的生理过程至关重要.
研究的目的:
- 介绍一个详细的实验协议,用于NTS的体内两光子成像.
- 为了能够在NTS中高分辨率记录神经人口活动.
- 促进对NTS神经元的功能多样性的研究.
主要方法:
- 对于小鼠的头骨窗口植入的最小侵入性手术程序.
- 在体内使用双光子显微镜进行神经成像.
- 在麻醉小鼠的NTS中记录细胞分辨率神经群活动的记录.
主要成果:
- 成功实施了NTS成像的逐步协议.
- 在NTS中证明了细胞水平的神经活动记录.
- 建立一种研究NTS神经元异质性的方法.
结论:
- 描述的协议为神经科学研究提供了有价值的工具.
- 这种技术可以对NTS神经回路进行详细的研究.
- 阐明NTS神经元机制可以促进对情绪和新陈代谢的理解.
相关概念视频
01:20Anatomy of the Brain: Major Regions
11.4K
The brain is the most complex organ in the human body. It consists of four main parts: the cerebrum, diencephalon, cerebellum, and brainstem.
The cerebrum is the largest section of the brain and divides into left and right hemispheres, separated by a deep fissure. The cerebral outer layer of grey matter — the cerebral cortex — comprises elevations called gyri and shallow groves called sulci. The inner portion of white matter includes long nerve fibers known as axons, which connect...
The cerebrum is the largest section of the brain and divides into left and right hemispheres, separated by a deep fissure. The cerebral outer layer of grey matter — the cerebral cortex — comprises elevations called gyri and shallow groves called sulci. The inner portion of white matter includes long nerve fibers known as axons, which connect...
11.4K
01:14Brain Imaging
1.0K
Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans), magnetic resonance imaging (MRI), functional magnetic resonance imaging (fMRI), and Transcranial Magnetic...
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans), magnetic resonance imaging (MRI), functional magnetic resonance imaging (fMRI), and Transcranial Magnetic...
1.0K