相关实验视频

Updated: Jan 9, 2026

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

Published on: July 18, 2025

低氧会诱导内菌Flavobacterium sp.的表型和代谢变化. 第九百九十八章没有了

Xinya Pan^1,2, Janne J Hageman³, Daan A Weits³

¹Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), 6708 PB Wageningen, The Netherlands.

The ISME journal

|December 6, 2025

概括

植物根中的内菌体验氧气不足,改变它们的行为. 这项研究揭示了这些低氧条件如何影响细菌特征和与植物病原体的相互作用.

科学领域:

微生物学微生物学
植物与微生物的相互作用
植物生理学植物生理学

背景情况:

氧气的可用性对于微生物生命至关重要.
内生菌细菌生活在植物组织中,面临着变化的氧气水平.
植物内气层中的低毒性状况尚不清楚.

研究的目的:

为了量化甜菜根组织中的氧气水平.
为了研究内菌 *Flavobacterium* sp. 的表型和代谢反应. 98到低氧气. 没有氧气.
了解低氧气如何影响 *Flavobacterium* sp.之间的相互作用. 98 和植物病原体 *Rhizoctonia solani*.

主要方法:

微传感器测量以确定甜菜根中的氧气梯度.
培养 *Flavobacterium* sp. 的植物. 在受控的低氧 (100 ppm) 和环境条件下.
现型分析 (生长,运动,殖民地扩散).
表甲基质组分析以分析细胞外代谢物.
对抗*Rhizoctonia solani*的抗真菌活性的评估.

主要成果:

氧气水平从根表皮显著下降到血管系统.
* 杆菌 * sp. 的存在. 98表现出增长减缓,但增强了运动性和殖民地在低氧条件下扩散.
低氧条件改变了细胞外代谢物概况,增加了lysophosphatidylethanolamine (lysoPE) 和N-乙-氨,同时减少了5,6-二甲基胺醇.
* 杆菌 * sp. 的存在. 在低氧条件下更有效地抑制了Rhizoctonia solani*的生长.

结论:

内生菌细菌在对低毒环境的反应中表现出显著的生理和代谢可塑性.
低氧触发了 *Flavobacterium* sp. 中的利基适应策略. 98,增强其对抗病原体的潜在作用.
这些发现强调了氧度梯度在塑造内生菌微生物群落及其在植物中的功能方面的重要性.

关键词:

这种细菌名为Flavobacterium.缺氧缺氧是指缺氧的情况.植物内植物相互作用.根部氧气测量根部氧气测量没有目标的代谢学.

更多相关视频

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica

Published on: September 20, 2024

Anaerobic Growth and Maintenance of Mammalian Cell Lines

Anaerobic Growth and Maintenance of Mammalian Cell Lines

Published on: July 21, 2018

相关概念视频

Oxygen Requirements and Growth Patterns

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...

Hypoxia

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...

Other Stress Responses in Bacteria

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...

Hyperthermophilic Bacteria

Hyperthermophilic Bacteria

Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...

Microbial Nutrition

Microbial Nutrition

Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...