挪威土壤样本的法医地理定位使用微生物组的随机森林分析
Aurora Hofsvang1, Eirik Natås Hanssen2, Eric Jacques de Muinck3
1Department of Forensic Sciences, Oslo University Hospital, Oslo, Norway; Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biosciences, University of Oslo, Oslo, Norway.
Forensic science international. Genetics
|December 6, 2025
概括
土壤微生物组可以确定地理来源,帮助法医调查. 位置显著影响微生物组成,但季节性变化可能会随着时间的推移影响准确性.
科学领域:
- 法医科学 法医科学 法医科学
- 微生物学 微生物学
- 生物信息学是一种生物信息学.
背景情况:
- 土壤的无处不在和粘性使其成为一种有价值的法医痕迹证据.
- 准确地确定土壤起源可以将个人或物体与特定位置联系起来.
- 土壤中的微生物群落提供了独特的地理标记的潜在来源.
研究的目的:
- 评估土壤微生物组在确定地理来源 (来源) 的潜力.
- 评估服装上的土壤污点样本作为土壤来源的代理的可靠性.
- 调查季节性变化对土壤微生物组合和来源准确性的影响.
主要方法:
- 从挪威奥斯陆的15个地点收集了土壤和模拟土壤污点样本,多次.
- 使用16S rRNA基因安普利康序列 (V4区域) 分析微生物组成.
- 应用机器学习算法来根据地理来源对样本进行分类.
主要成果:
- 微生物组的组成与地理位置有很强的相关性,对于土壤和土壤染色样品.
- 在一周内采集的样本中,分类准确度超过85%.
- 季节性变化显著影响了微生物组的组成,将不同季节的样本的精度降低到55-64%.
结论:
- 土壤微生物组是法医土壤来源确定的一个有希望的指标.
- 位置是土壤微生物社区结构的主要驱动因素.
- 必须考虑时间变化,特别是季节性变化,以便在法医中进行可靠的土壤微生物组分析.
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