相关实验视频

Updated: Jan 9, 2026

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Published on: February 10, 2015

通过 Decoy oligodeoxynucleotide 准 X 盒结合蛋白-1 调节激活的肝星细胞的纤维性特征

Roya Solhi¹, Majid Lotfinia², Zahra Abdi³

¹Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran; Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research, Tehran, Iran.

The Journal of pharmacology and experimental therapeutics

|December 6, 2025

概括

准X盒结合蛋白-1 (XBP1) 用诱性寡氧核酸 (ODN) 抑制激活的肝星细胞 (aHSC) 激活. 这种方法显示了通过调节内质网膜应激和纤维基因表达来治疗肝纤维化的前景.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
肝病学肝病学是一种肝病学.

背景情况:

活化的肝星细胞 (aHSCs) 是肝纤维化发展的核心.
细胞内膜网膜 (ER) 的压力,特别是通过需要内醇的酶1 (IRE1) -X盒结合蛋白-1 (XBP1) 途径,驱动HSC激活.
针对aHSCs是一个潜在的肝纤维化治疗策略.

研究的目的:

为了研究XBP1特异性诱寡氧核酸 (ODN) 在调节aHSC激活中的有效性.
评估XBP1诱ODN对激活HSC中的关键纤维素基因和蛋白质表达的影响.

主要方法:

使用转化生长因子β (TGF-β) 激活LX-2细胞.
使用生物信息学工具 (JASPAR,CLC主工作台) 设计了XBP1特定的诱ODN序列.
在ODN治疗后分析了基因表达 (定量RT-PCR),蛋白质水平 (免疫阻塞) 和原分泌 (ELISA).

主要成果:

使用XBP1诱ODN的治疗显著降低了激活LX-2细胞中的lysyl氧化酶的mRNA表达,矩阵金属蛋白酶的组织抑制剂,α-平滑肌肉动蛋白和纤维素.
免疫清洗证实了α-平滑肌肉actin的蛋白质表达减少.
与对照细胞相比,ELISA在ODN治疗的细胞中显著减少了原分泌.

结论:

XBP1诱ODN有效地抑制肝星细胞激活.
通过XBP1向调节ER压力和纤维素通路,为肝纤维化提供了一种新的治疗方法.
这一战略有望为慢性肝病开发创新治疗方法.

关键词:

激活的肝细胞星形细胞.原蛋白分泌的分泌物肝脏纤维化肝脏纤维化 X-box结合蛋白-1诱的寡氧核酸.

更多相关视频

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Published on: December 21, 2019

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Published on: March 11, 2017

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...