用酶辅助从全粉和剥皮面粉中提取海军豆蛋白:对提取能力,结构和技术功能性质的影响
Md Junaeid Khan1, Sindhu Chaudhary2, Amanat Ali2
1Department of Interdisciplinary Engineering, College of Engineering, University of Guelph, Guelph, ON, N1G 2W1, Canada; Department of Food Engineering, Dhaka University of Engineering & Technology, Gazipur, Gazipur, 1707, Bangladesh.
International journal of biological macromolecules
|December 6, 2025
概括
酶辅助提取显著改善了海军豆蛋白质的质量和功能. 这种绿色方法提高了蛋白质的溶解性,乳化和水的吸收,使海军豆成为更可行的可持续蛋白质来源.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 可持续农业 可持续农业
背景情况:
- 对可持续植物蛋白质的日益增长的需求需要改进的提取方法.
- 海军豆提供低过敏性,无质蛋白质,但由于常规提取限制,其利用率不足.
- 目前的方法往往导致蛋白质变质和聚合,减少功能性质.
研究的目的:
- 评估海军豆粉的酶辅助 (E) 和同质化酶辅助 (HE) 提取方法.
- 将这些方法与传统的水性提取 (Aq) 进行比较.
- 评估提取对蛋白质提取能力,结构和技术功能性质的影响.
主要方法:
- 酶辅助 (蛋白酶,pH10,0.5%,1.5h) 和同质化酶辅助提取整个和剥皮的海军豆面粉.
- 与水性提取进行比较.
- 分析蛋白质纯度,回收,可溶性,乳化特性 (EAI,ESI),水/油吸收 (WAC,OAC),凝 (LGC),结构变化 (SEM,FTIR,XRD,DSC,光谱学) 和自由硫含量.
主要成果:
- 同质化酶辅助脱皮 (HE-D) 面粉具有最高的蛋白质纯度 (68.99%).
- 酶辅助脱皮 (E-D) 和HE方法产生了最高的蛋白质回收.
- 基于酶的方法提高了溶解度,乳化活性指数 (EAI) 和吸水能力 (WAC).
- 结构分析揭示了蛋白质展开和破坏聚合物,增强功能性质.
结论:
- 基于酶的提取是一种可行的绿色方法,可以提高海军豆蛋白质的质量.
- 这些方法改善了食品应用的结构和技术功能性质.
- 优化提取可以提高海军豆作为可持续蛋白质来源的利用率.
相关概念视频
01:18Detergent Purification of Membrane Proteins
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
01:24DNA Isolation
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...