细胞外矩阵的物理特性调节3D水凝微组织中的癌细胞形态转变
Ayda Pourmostafa1, Gabrielle Uskach1, Mohammad Jafari2
1Department of Biomedical Engineering, New Jersey Institute of Technology, Newark, NJ, USA.
Acta biomaterialia
|December 6, 2025
概括
固体瘤细胞形态是由细胞外矩阵 (ECM) 机制调节的. 细胞在柔软的ECM中采用延长的形状,在刚硬的ECM中采用集群的形状,影响入侵. 这为更好的癌症模型提供了信息.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 固体瘤细胞表现出与进展相关的多种形态.
- 对细胞外基质 (ECM) 的反应影响细胞形态的因素尚不清楚.
- 了解这些因素对于建模瘤入侵和开发向疗法至关重要.
研究的目的:
- 研究细胞外矩阵 (ECM) 机制如何在3D中调节固体瘤细胞形态.
- 开发和验证基于ECM特性预测细胞形态的理论模型.
- 建立一个强大的实验平台,研究三阴性乳腺癌 (TNBC) 细胞中的细胞-ECM相互作用.
主要方法:
- 根据最低能量的原则,开发了一种理论机械能量模型.
- 使用的MDA-MB-231乳腺癌细胞被封装在控制硬度的3D水凝中.
- 采用化学上不同的,但机械上匹配的复合材料矩阵来隔离物理效应.
主要成果:
- 理论模型预测了细胞形态,最大限度地减少了总细胞-ECM系统能量.
- 试验室实验证实了模型的预测:软ECM中的延长形态,硬ECM中的集群形态.
- ECM的刚性,而不是化学性质,控制了细胞形态过渡.
结论:
- 在很大程度上,ECM的物理特性决定了固体瘤细胞的形态和入侵行为.
- 该研究提供了细胞入侵的生物物理模型和TNBC研究的平台.
- 这些发现对个性化医疗和量身定制的癌症模型的开发有影响.
更多相关视频
相关概念视频
02:17The Tumor Microenvironment
7.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.6K
01:29The Extracellular Matrix
11.8K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
11.8K
01:42The Extracellular Matrix
88.0K
Overview
88.0K
01:13Cell-matrix's Response to Mechanical Forces
3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
3.4K