在工业大豆粉生产过程中,非挥发性代谢物的变化趋势是通过非向的代谢学来实现的
Chengtuo Niu1, Shuhe Chen1, Wenjun Zuo2
1Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Lab of Brewing Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Food research international (Ottawa, Ont.)
|December 6, 2025
概括
这项研究揭示了工业性豆 (BBP) 发酵中使用代谢学的非挥发性代谢物. 确定了关键的代谢途径和75种功能代谢物,提高了对BBP生产的理解.
科学领域:
- 食品科学与技术 食品科学与技术
- 代谢学 代谢学 代谢学
- 发酵科学 发酵科学
背景情况:
- 基于豆 (BBP) 是一种广泛消费的发酵调料.
- 之前关于BBP发酵的研究主要集中在微生物群落和风味特征上.
- 工业BBP生产期间的非挥发性代谢场景在很大程度上仍然没有特征.
研究的目的:
- 综合分析工业BBP发酵过程中非挥发性代谢物的组成和动态变化.
- 确定影响BBP质量的关键代谢途径和特定代谢物.
- 提供对大规模BBP生产过程中发生的生物化学转变的见解.
主要方法:
- 采用非向的代谢学方法来分析BBP中的非挥发性代谢物.
- 利用KEGG数据库进行综合路径分析.
- 应用OPLS-DA和随机森林分析以确定差异化代谢物.
主要成果:
- 在BBP中共鉴定了1992个非挥发性代谢物.
- 氨基酸代谢 (27.94%) 和二次代谢物生物合成 (14.29%) 被确定为主要的代谢途径.
- 碳水化合物和氨基酸代谢在初始和中期发酵阶段得到丰富,而二次代谢物合成在中后期阶段占主导地位.
- 预测了具有潜在生理功能的75种差异性代谢物,并绘制了它们的变化趋势.
结论:
- 这项研究阐明了工业BBP发酵的复杂非挥发性代谢组.
- 代谢途径的转变与不同的发酵阶段相关,影响BBP特征.
- 已识别的功能代谢物为了解和优化BBP生产过程提供了基础.
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