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单链可变片段基于点块,单个和多个测试,用于快速SARS-CoV-2诊断

Dilek Çam Derin¹, Enes Gültekin², Irmak İçen Taşkın¹

¹Inonu University, Department of Molecular Biology and Genetics, 44280 Malatya, Turkey.

Journal of AOAC International

|December 6, 2025

概括

这项研究开发了一种新的单链可变片段 (scFv),用于使用侧流试验 (LFA) 快速检测病毒. 将scFv与aptamers结合起来,在临床样本中改善了SARS-CoV-2的检测.

科学领域:

生物技术是生物技术.
免疫学免疫学免疫学
分子诊断学分子诊断

背景情况:

快速诊断测试对于管理流行病至关重要,提供了显著的时间优势.
侧流检测 (LFA) 是实用的,无设备的诊断工具,通常使用抗体对.
在SARS-CoV-2的流行突出了有效的病毒检测方法的需要.

研究的目的:

通过重组产生单链可变片段 (scFv),以向SARS-CoV-2尖端受体结合域 (sRBD).
使用scFv结合抗体和阿帕特默对开发LFA,包括一个阿帕特默尾酒.
评估这些新型试验格式的诊断性能.

主要方法:

复合生产的SARS-CoV-2 特定的 scFv.
使用金纳米粒子作为标签的三明治测试的开发.
使用scFv,CR3022抗体和对S和N蛋白的aptamers在LFA,Dot Blot Assay (DBA) 和多重格式.

主要成果:

在高度下产生生物活性scFv.
通过LFA,DBA和多重检测,可以有效检测病毒.
DBA和多重LFA成功地在患者拭子样本中识别了SARS-CoV-2,在合成标上表现优于单个LFA.

结论:

当scFv与aptamer结合时,可以在三明治试验中增强标结合,这是一个新发现.
不同的测试配置和捕获剂组合影响scFv的有效性.
多种测试策略和新的组合提供了改进的病毒诊断和经济有效的快速测试.

关键词:

这是点点测试.有多个LFA.这就是SARS-CoV-2病毒.scFvv 在线阅读一个单个LFAFA.

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