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Updated: Jul 9, 2026

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
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Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

Published on: November 29, 2013

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使用-交换质谱法分析粉样纤维的结构分析协议

Qian Meng1, Yuliang Han2, Hu Zhou1

  • 1Department of Analytical Chemistry, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

STAR protocols
|December 7, 2025
PubMed
概括

这项研究详细介绍了一种-交换质谱法 (HDX-MS) 协议,用于分析粉样蛋白结构,以帕金森病相关的α-synuclein纤维素作为模型. 该方法有助于理解不溶性蛋白质聚合物.

关键词:
质谱测量质量谱测量蛋白质的表达和净化过程结构生物学 结构生物学

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Amyloid Fibrils03:03

Amyloid Fibrils

Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
Amyloid deposits were observed as early as 1639 in the liver and the spleen.   In 1854, Rudolph Virchow performed iodine staining, normally used to...