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在血和上皮层内膜液中测量聚米辛E的量化:验证和临床应用

Shengnan Zhang¹, Ruwei Yang¹, Hongyi Tan²

¹Department of Pharmacy, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, China.

Journal of pharmaceutical and biomedical analysis

|December 7, 2025

概括

对于治疗耐药性感染而言,聚米辛E (PME) 监测至关重要. 一种新的LC-MS/MS方法准确地测量患者液体中的PME,有助于个性化治疗并揭示损伤联系.

科学领域:

药理学药理学是指药理学的学科.
临床化学临床化学
传染性疾病传染性疾病

背景情况:

聚米辛E (PME) 是治疗多药耐药性格拉姆阴性感染的关键抗生素.
它狭窄的治疗窗口和高的药理动力学变异性需要治疗药物监测 (TDM).

研究的目的:

开发和验证一种敏感的LC-MS/MS方法,用于量化等离子体和上皮层层液 (ELF) 中的PME.
评估该方法在重症患者中的临床实用性.

主要方法:

开发并验证了一种LC-MS/MS方法,用于在血和ELF中量化 PME.
分析了来自40名重症患者的血样本和接受胆固醇硫酸盐治疗的4名患者的配对血/ELF样本.
根据FDA/EMA指南验证了该方法.

主要成果:

这种LC-MS/MS方法表现出对常规TDM的敏感性和适用性.
在急性损伤患者中,PME的血最低度显著更高.
与血相比,吸入胆固醇硫酸盐的使用导致了ELF中更高的PME度.

结论:

经过验证的LC-MS/MS方法适用于Polymyxin E.的常规TDM.
低血水平可能与严重病患者的急性损伤有关.
吸入后ELF度提供了肺部药物暴露的证据,支持针对个性化治疗的进一步研究.

关键词:

皮质内膜液体皮质内膜液体这是LC-MS/MS.血等离子体的使用方法波利米克辛 E 是一个多重物质.治疗药物监测治疗药物监测

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