大肠杆菌的核酶域 RecBCD 酶调节DNA结合和结基对
Linxuan Hao1, Rui Zhang1, Timothy M Lohman1
1Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S. Euclid Avenue, Box 8231, Saint Louis, MO 63110, United States.
Journal of molecular biology
|December 7, 2025
概括
这是RecBCD酶.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- RecBCD是E. coli中双链DNA断裂修复中的一个关键酶.
- 它具有酶和核酶活动,在RecB和RecD中具有运动域,并在RecB中具有核酶域 (RecBNuc).
研究的目的:
- 研究RecB核酶域在RecBCD的DNA结合和解过程中的作用.
- 为了比较RecBCD的DNA结合特性和缺乏核酶域的RecBΔNucCD变体.
主要方法:
- 对DNA结合亲和力和热力学进行比较分析.
- 用冷电子显微镜 (cryo-EM) 确定与DNA结合的RecBCD和RecBΔNucCD的结构.
- 热力学测量 (ΔHobs) 的DNA结合.
主要成果:
- RecBCD与的DNA末端的结合是以热驱动的.
- 删除RecB核酶域 (RecBΔNucCD) 增加了DNA结合亲和力,表明了全效应.
- 与RecBCD (11 bp) 相比,RecBΔNucCD在结合形DNA末端时化了更少的基对 (4 bp).
结论:
- 在RecB核酶域的整体调节的基对融化的程度由RecBCD.
- RecBNuc可能通过RecB和RecC链接器之间的相互作用来发挥其长距离的异质效应.
更多相关视频
10:36Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
Published on: December 21, 2010
11.1K
05:33Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
Published on: July 5, 2024
1.3K
相关概念视频
01:20Mismatch Repair
6.2K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
6.2K
01:36Mismatch Repair
43.5K
Overview
43.5K
00:55DNA Helicases
23.8K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
23.8K
01:38Nucleotide Excision Repair
4.9K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
4.9K
01:08Nucleotide Excision Repair
40.5K
Overview
40.5K
02:31Homologous Recombination
62.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.4K