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在不同温度条件下90天内对SARS-CoV-2RNA稳定性的-MMTM分子介质的评估

Giasemi C Eptaminitaki¹, Alexandros Zafiropoulos¹, George Sourvinos¹

¹Laboratory of Clinical Virology, Medical School, University of Crete, Heraklion, Crete 71003, Greece.

Journal of virological methods

|December 7, 2025

概括

-MMTM分子介质在室温下有效保持SARS-CoV-2的稳定性,长达90天. 这种经过验证的运输介质可以确保COVID-19测试的准确诊断结果,而不需要冷藏.

科学领域:

临床诊断临床诊断临床诊断
病毒学病毒学
微生物学微生物学

背景情况:

准确的临床样本采集和储存对于可靠的诊断结果至关重要.
片是收集微生物检测和识别样本的常见工具.
在运输介质中SARS-CoV-2的稳定性对于准确的COVID-19诊断至关重要.

研究的目的:

评估-MMTM分子介质在保持SARS-CoV-2稳定性的有效性.
评估各种储存温度和持续时间对SARS-CoV-2RNA完整性的影响.
为了确定-MMTM是否能够在环境条件下可靠地运输和储存样本.

主要方法:

42个SARS-CoV-2阳性样本被收集并储存在-MMTM中.
标本受到各种储存条件的影响:4°C/7天,室温 (RT)/7天,-80°C/1个月,RT/1个月,-80°C/3个月,RT/3个月.
使用实时PCR量化SARS-CoV-2RNA水平.

主要成果:

与基线相比,在所有测试的储存条件中没有观察到SARS-CoV-2RNA水平的统计学上显著差异.
-MMTM证明了SARS-CoV-2RNA在环境温度下有效保存长达90天.
CT值保持稳定,表明病毒RNA没有显著降解.

结论:

-MMTM分子介质是SARS-CoV-2的可靠传输介质.
使用-MMTM在环境条件下储存和运输样本不会影响诊断的准确性.
进一步的研究将调查-MMTM的延长储存时间.

关键词:

分子运输介质是分子运输介质.在SARS-CoV-2的RNA稳定性方面.标本的储存条件标本的储存条件

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