用计算分散光成像和极度度二次子显微镜揭示了含原体的组织中的纤维方向
Loes Ettema1, Viktoras Mažeika2,3, Mehdi Alizadeh2,4,5,6
1Department of Imaging Physics, Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands. L.Ettema@tudelft.nl.
Scientific reports
|December 7, 2025
概括
计算机分散光成像精确地绘制了组织中的原纤维方向. 这种技术与极度度二次子生成显微镜进行了验证,对各种原样本显示了类似的结果.
科学领域:
- 生物医学工程 生物医学工程
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
背景情况:
- 原纤维组织对于组织机制和功能至关重要.
- 需要精确的成像技术来可视化密集的原蛋白网络.
- 计算分散光成像 (CSLI) 提供快速光纤定向检索,但需要验证.
研究的目的:
- 通过使用CSLI在原组织中量化验证平面内纤维的方向.
- 为了比较CSLI结果与极度二次波生成 (pSHG) 显微镜.
- 评估CSLI在各种样本类型和条件上的性能.
主要方法:
- 在老鼠肌和骨段上对CSLI和pSHG显微镜进行比较分析.
- 对有染色和没有染色的切口,折叠的层和斜切口的调查.
- 两种技术之间的纤维方向相似性的定量评估.
主要成果:
- 通过CSLI和pSHG显微镜,在老鼠肌和骨中,产生了相似的纤维方向.
- 在肌的内平面,单向纤维中观察到最高的相似性.
- CSLI成功地恢复了折叠肌中的多向纤维的方向,不受染色的影响.
结论:
- CSLI是一种经过验证的技术,用于定量评估原纤维方向.
- CSLI提供了具有微米分辨率的大视野测量.
- pSHG显微镜提供了高分辨率的超结构原信息,补充了CSLI.
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