转录因子 SlJIG 调节番茄对入侵性害虫 Phthorimaea absoluta 的多层防御
Zhiwei Kang1,2, Xiaoyan Guo1, Zichao Song1
1College of Life Science/Hebei Basic Science Center for Biotic Interactions, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
The New phytologist
|December 8, 2025
概括
番茄植物中的Sljig基因调节了对入侵性害虫Phthorimaea absoluta的防御. 修改Sljig会增加害虫的易感性,改变捕食者的吸引力,影响番茄的耐药性.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 化学生态化学生态学
背景情况:
- 基本氨酸拉链 (bHLH) 转录因子对于植物抗压能力至关重要.
- 对于bHLH转录因子对番茄防御Phthorimaea absoluta的作用尚不清楚.
研究的目的:
- 调查 bHLH 转录因子 SlJIG 在番茄防御 Phthorimaea absoluta 的作用.
- 阐明SlJIG介导的抗性背后的分子机制.
主要方法:
- 基因表达分析作为对P. absoluta感染,甲基斯酸盐和伤害的反应.
- 在番茄植物中Sljig的基因编辑.
- 评估植物对P. absoluta幼虫的敏感性和卵位偏好.
- 对捕食者吸引力的分析 (Nesidiocoris poppiusi).
- 代谢物概况和生物合成途径的分析 (α-托马丁,黄,烯).
主要成果:
- SlJIG的表达是由P. absoluta的感染,甲基斯酸盐和伤害引起的.
- SlJIG基因编辑增加了番茄对P. absoluta的易感性,并减少了自然捕食者N. poppiusi的吸引力.
- SlJIG激活了α-托马丁和黄类生物合成途径中的关键酶,并抑制了合成.
- 基因编辑Sljig降低了α-托马丁和kaempferol水平,并改变了α-humulene排放.
结论:
- SlJIG在调节番茄防御机制对抗绝对的过程中发挥着关键作用.
- SlJIG 影响关键防御代谢物和挥发性有机化合物的生物合成.
- SlJIG代表了培育增强番茄对这种入侵性害虫的耐药性的潜在目标.
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