乌尔瓦乳的细胞外囊泡通过Nrf2/Keap1/HO-1激活来保护高脂肪饮食引起的肠损伤
Jiaxuan Li1,2, Yu Li1,2, Xunyu Song1,2
1State Key Laboratory of Marine Food Processing and Safety Control, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, China. suwentao2020@yeah.net.
Food & function
|December 8, 2025
概括
超细胞囊 (UEVs) 保护肠道屏障. 这些新的纳米结构降低了氧化应激,改善了肠道完整性,为向代谢肠道疾病的功能性食物提供了潜在的潜力.
科学领域:
- 海洋生物技术 海洋生物技术
- 营养学科学 营养学科学
- 胃肠病学 胃肠病学
背景情况:
- 高脂肪饮食 (HFDs) 损害了肠道屏障功能,增加了透性和氧化应激,与与肥胖相关的疾病有关.
- 乌尔瓦乳细胞外囊泡 (UEVs) 是未被充分探索的纳米结构,具有潜在的功能性食品应用.
研究的目的:
- 研究来自Ulva lactuca的UEVs的治疗潜力,以减轻HFD引起的肠损伤.
- 描述UEV并评估它们对氧化应激,亡和肠道屏障完整性的影响.
主要方法:
- 来自Ulva lactuca. 的UEVs的分离和表征.
- 在体外测试中评估ROS水平,亡和紧结蛋白表达 (ZO-1,Occludin).
- 在HFD条件下评估TEER和粘素分泌的肠上芯片模型.
- 涉及Nrf2/Keap1/HO-1信号通路的机制研究.
主要成果:
- UEVs表现出杯状形态,平均直径为188.8nm.
- UEV治疗显著降低了41.77%的ROS和11.31%的细胞亡,恢复了紧结蛋白.
- 在肠上芯片模型中,UEV在HFD压力下增加了TEER的1.14倍,并增强了粘素分泌.
- UEVs激活了Nrf2/Keap1/HO-1通路,促进了抗氧化基因表达.
结论:
- 在减少氧化应激和保护肠道屏障方面,UEV具有双重功能.
- 这些发现支持UEV作为功能性食品的新型食品衍生纳米结构的潜力,旨在治疗代谢性肠道疾病.
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