来自乳酸菌的后生物的宽谱抗菌活性Lacticaseibacillus paracasei BGP1对抗多抗药性皮肤伤口病原体
Farideh Mohammadhosseinzadeh1, Ehsan Arefian1, Mouj Khaleghi2
1Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
MicrobiologyOpen
|December 8, 2025
概括
来自Lacticaseibacillus paracasei BGP1的后生物体显示出作为治疗皮肤伤口感染的抗生素稳定替代品的希望. 这些无生命的化合物有效地对抗常见的皮肤病原体,如金黄色葡萄球菌和 Pseudomonas aeruginosa.
科学领域:
- 微生物学 微生物学
- 皮肤病学 皮肤病学
- 生物技术是生物技术.
背景情况:
- 抗生素耐药性需要针对皮肤伤口感染的新型治疗策略.
- 益生菌具有潜力,但在皮肤病学中面临着活细胞的配方挑战.
- 后生物质为抗微生物应用提供了稳定,安全和无生命的替代品.
研究的目的:
- 评估从益生菌菌株,特别是Lacticaseibacillus paracasei BGP1.1中获得的后生菌的抗菌潜力.
- 评估无细胞超级生物 (CFSs) 对临床相关皮肤病原体的疗效.
- 为了确定负责观察到的抗微生物活性的生物活性化合物.
主要方法:
- 从六种益生菌菌株中选CFS,使用阿加井扩散和汁微稀释试验.
- 对金黄色葡萄球菌 (ATCC 25923),MRSA (UTMC 1442), Pseudomonas aeruginosa (ATCC 27853) 和PAO1.1进行检测.
- 气色谱-质谱 (GC-MS) 用于生物活性化合物的鉴定.
主要成果:
- 乳酸菌 (Lacticaseibacillus paracasei BGP1) 显示出显著的抗菌活性,最小抑制度 (MIC) 为6.25 mg/mL,最小杀菌度 (MBC) 为12.5 mg/mL.
- 在GC-MS分析中,发现棕酸 (33.24%) 和酸 (46.45%) 是 BGP1 CFS 中的主导生物活性化合物.
- 来自BGP1的CFS对测试的皮肤病原体表现出广泛的活性.
结论:
- 来自L. paracasei BGP1的后生物代谢物对抗生素耐药性皮肤伤口病原体有效.
- 这些发现突出了对传统抗生素疗法的有希望,稳定和无生命的替代方案.
- 进一步的体内研究是有必要的,以探索治疗潜力和临床配方.
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