相关实验视频
Updated: Jan 9, 2026

10:37
Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
36.4K
康尼辛通过双域机制抑制EMT,以保持上皮质身份
Bo Ma1,2, Zhaodan Ding1,2, Guangyan Wang1,2
1Department of Biochemistry and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78229-3900.
Research square
|December 8, 2025
概括
康尼辛50 (Cx50) 抑制了表皮-介质细胞过渡 (EMT),这是一个驱动纤维性疾病和白内障手术后视力丧失的过程. Cx50 Cx50 的意思是
科学领域:
- 细胞生物学 细胞生物学
- 眼科医生 眼科 眼科
- 纤维化研究 纤维化研究
背景情况:
- 皮质-介质细胞过渡 (EMT) 驱动纤维性疾病和后囊模糊化 (PCO),是视力丧失的原因.
- 控制上皮质完整性和EMT的分子机制尚未完全理解.
- 已知连接因通过间隙结进行细胞间通信.
研究的目的:
- 确定EMT的新型调节器.
- 研究连因素50 (Cx50) 在调节EMT中的作用.
- 探索Cx50的功能超出了间隙结的作用.
主要方法:
- 使用小和小鼠模型进行体外,体外和体内研究.
- 转化生长因子-β2 (TGF-β2) 治疗以诱导EMT.
- Cx50过度表达和淘汰赛小鼠模型.
- 分析EMT标记物 (α-平滑肌肉动蛋白,纤维素) 和蛋白质复合物 (β-catenin,E-cadherin).
主要成果:
- TGF-β2降低了Cx50的调节,诱导了EMT标记物;Cx50的过度表达逆转了这些变化.
- Cx50的细胞外E2域限制了表皮细胞迁移和EMT.
- Cx50的C端域稳定了β-catenin-E-cadherin复合体,防止了EMT.
- 在Cx50淘汰赛小鼠中,透镜提取后的EMT呈现加速.
结论:
- Cx50是一种新的,EMT和上皮质可塑性的双域调节器.
- Cx50的功能超出了其在间隙连接中的规范性作用.
- Cx50对治疗眼睛和其他上皮组织中的纤维性疾病有意义.
相关概念视频
Cadherins in Tissue Organization
3.8K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
3.8K
Tight Junctions
6.8K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
6.8K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.6K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.6K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.4K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K
Cell Motility through Blebbing
2.4K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
2.4K
Tension Response at Adherens Junctions
3.4K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
3.4K

