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集成基于磁珠的SELEX与In Silico结合分析,用于识别针对TAGLN2的高 afinity DNA Aptamers

Darius R Martin^1,2, Ayman Waddad³, Mohammed A Gafar⁴

¹Department of Science, Technology and Innovation/TIA Nanotechnology Platform, Department of Biotechnology, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville, Cape Town 7535, South Africa.

|December 8, 2025

概括

研究人员确定了高亲和度DNA吸收体,向转林-2 (TAGLN2),这是癌症等疾病的生物标志物. 这些用SELEX和计算方法选择的aptamer显示出开发早期疾病检测新诊断工具的前景.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
生物标志物发现发现

背景情况:

转基因-2 (TAGLN2) 涉及到细胞压力,并作为潜在的癌症和结核病等疾病的诊断生物标志物.
开发TAGLN2的高亲和度阿马对于推进诊断能力至关重要.

研究的目的:

为了识别和表征具有高结合亲和力转林-2 (TAGLN2) 的DNA吸收体.
评估这些体的潜力,以开发TAGLN2相关疾病的诊断工具.

主要方法:

通过指数式丰富 (SELEX) 的连接体的系统演化被用来选择针对重组TAGLN2.2的体.
使用了DNA序列分析,遗传学分析,二次结构预测 (M-fold),微尺度热泳 (MST) 和in silico分子对接/动力学模拟.
用解离常数 (Kd) 量化结合亲和力,并通过计算建模验证.

主要成果:

鉴定出了针对TAGLN2的十个独特的阿普坦酶序列.
7和9的aptamers证明了对TAGLN2的验证结合,分别具有18 ± 1 nM和50 ± 2 nM的解离常数.
在分析表明,胺基1可能具有最高的结合亲和力,其次是胺基7,支持实验发现.

结论:

与SELEX集成in silico分析是一种有效的策略,用于识别高亲和度的体.
已识别的TAGLN2向性受体对开发用于早期疾病检测的新型诊断试验具有重大潜力.

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