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通过比较性转录学揭示了甘对Pratylenchus zeae的多层防御反应

Pedro M S Confort¹, Tiarla Graciane Souto^1,2, Jonathan F Macedo¹

¹Departamento de Genética, Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Esalq, Piracicaba, SP, Brazil.

Frontiers in plant science

|December 8, 2025

概括

甘对根损伤线虫 Pratylenchus zeae 的耐药性涉及复杂的,多层次的防御. 耐药品种表现出早期的代谢重编程和细胞壁强化,与反应延迟的敏感品种不同.

科学领域:

植物病理学植物病理学
分子遗传学分子遗传学
农业科学农业科学

背景情况:

根损伤线虫 (Pratylenchus zeae) 是对甘生产的主要威胁.
甘对P. zeae耐药性的分子机制尚不清楚.

研究的目的:

调查甘对Pratylenchus zeae的耐药性的分子基础.
为了比较耐药和易感的甘品种的根转录基因反应.

主要方法:

甘根 (品种RB966928和CTC9001) 的比较转录组 15天后与P. zeae.进行注射.
对差异表达基因 (DEG) 和基因本体学 (GO) 丰富的分析.

主要成果:

耐药品种 (RB966928) 表现出协调的多层防御,包括加强的糖解,脂肪酸生物合成,ROS活性和莉花/酸通路的激活.
敏感品种 (CTC9001) 显示出延迟反应,细胞增殖和与相关的防御不足以限制线虫的进展.
基因本体学分析突出了耐药和易受反应之间的明显的代谢,信号和细胞壁组织差异.

结论:

持久抵抗P. zeae需要堆叠互补的防御层,而不是单一的机制.
已识别的候选基因 (RGA,PR1同类基因) 和途径为甘中线虫耐药性的标记器辅助育种和基因编辑提供了目标.

关键词:

普拉提伦丘斯海 (Pratylenchus zeae) 是一种海藻类.比较的转录组学进行比较植物病理学植物病理学植物类动物的相互作用.甘是一种糖,

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