可以激活CBASS免疫,保护细菌免受菌体感染
Xiao Wang1, Yongdong Li2, Xiao Wang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
mBio
|December 8, 2025
概括
(Mn2+) 通过增强基于周期性寡核酸的抗菌体信号系统 (CBASS) 来增强细菌对菌体的防御能力. 这种辅助因子增强了DncV的活性,导致快速的细胞溶解和减少菌体复制,反映了哺乳动物的抗病毒反应.
科学领域:
- 细菌天生的免疫力 细菌天生的免疫力
- 抗病毒防御的分子机制.
- 原核生物和真核生物的信号通路.
背景情况:
- 菌体对细菌构成持续的威胁,需要强大的防御系统,比如基于周期性寡核酸的抗体信号系统 (CBASS).
- 金属离子,特别是 (Mn2+) 在调节CBASS活性和细菌抗病毒防御中的作用在很大程度上是未知的,尽管它们已知对相关的真核细胞通路 (如cGAS-STING) 的影响.
研究的目的:
- 调查 (Mn2+) 作为细菌循环寡核酸基抗菌体信号系统 (CBASS) 中的辅因子的作用.
- 阐明Mn2+增强CBASS介导的抗病毒活性和细菌对菌体感染的耐药性的机制.
主要方法:
- 研究了Mn2+对DncV循环酶的活性及其3'3'-cGAMP的产生的影响.
- 评估了Mn2+增强的CBASS信号对脂酶CapV的激活和随后的细菌细胞溶解的影响.
- 在菌体感染期间检查了细胞内Mn2+水平和Mn2+载体基因表达的变化.
主要成果:
- (Mn2+) 直接增加了DncV循环酶的活性,显著增加了3'3'-cGAMP的产生.
- 菌体感染期间细胞内Mn2+水平升高加速了CapV的激活,导致细菌细胞过早溶解并减少了菌体的复制.
- Mn2+减轻了叶酸介导的DncV抑制,突出了其在调节循环二核酸信号传递和细菌抗病毒防御中的作用.
结论:
- (Mn2+) 是CBASS激活的关键辅因子,通过类似于哺乳动物cGAS-STING的保守机制增强细菌对菌菌体感染的抵抗力.
- 这项研究揭示了细菌天生的免疫的新方面,强调了Mn2+平衡在打击病毒威胁中的重要性.
- 这些发现为我们更深入地了解金属离子和循环二核酸信号在 prokaryotic 抗病毒防御中的相互作用.
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