对葡萄园相关细菌及其对抗 Botrytis cinerea 的抗作用进行了比较分析
Mahlatse Ellias Moremi1, Heinrich Wilbur du Plessis2, Marina Rautenbach3
1South African Grape and Wine Research Institute, Stellenbosch University, Private Bag X1, Stellenbosch, 7599, South Africa.
Journal of applied microbiology
|December 8, 2025
概括
研究人员确定了Bacillus物种和Pantoea聚合物,它们对Botrytis cinerea具有显著的抗真菌活性. 这种对葡萄团腐烂管理至关重要的细菌活动与特定的脂化合物有关.
科学领域:
- 农业微生物学 农业微生物学
- 植物病理学 植物病理学
- 生物控制剂 生物控制剂
背景情况:
- 葡萄团腐烂是由Botrytis cinerea引起的,对葡萄种植有很大的影响.
- 识别有效的生物控制剂对于可持续葡萄生产至关重要.
研究的目的:
- 隔离和描述葡萄酒环境中的具有抗真菌性质的细菌.
- 评估这些细菌对Botrytis cinerea的作用方式.
主要方法:
- 从葡萄藤土壤和叶子中分离了188种细菌菌株.
- 使用双培养试验对三种Botrytis cinerea菌株进行查.
- 确定了九种强效分离物 (Bacillus spp. 和Pantoea聚合物) 以及它们的抗真菌机制的特征.
主要成果:
- 九个分离物对Botrytis cinerea具有≥60%的抗真菌活性.
- 在低pH (pH5) 时,抗真菌活性降低.
- 奇丁酶的产生是常见的;在五个孤立体中观察到葡萄糖酶的产生.
- B. nakamurai菌株表现出强烈的子发芽和菌根生长的抑制,与伊图林,表面因子和素有关.
结论:
- 在Bacillus spp.中的变异性. 针对B. cinerea的抗真菌活性与脂型相关.
- 产生巴西洛米辛D的菌株显示出比产生伊图林A的菌株具有更高的功效.
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