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克服连接体发现挑战:开发SPSB2的基于的标记物

Christopher Lenz^1,2, Lewis Elson^1,2, Johannes Dopfer^1,2

¹Institute for Pharmaceutical Chemistry, Johann Wolfgang Goethe-University, Max-von-Laue-Straße 9, Frankfurt am Main 60438, Germany.

ACS chemical biology

|December 8, 2025

概括

开发细胞透 (CPP) 增强了用于E3酶连接体设计的基于极性降解子的分子的传递. 这一策略使强大的目标参与 (TE) 成为PROteolysis TTargeting Chimeras (PROTACs) 开发的关键.

科学领域:

生物化学生物化学
分子生物学分子生物学
药物发现药物发现药物发现

背景情况:

开发新的E3结合酶连接体对于像PROTEOLYSIS TTargeting Chimeras (PROTACs) 这样的异构分子至关重要.
评估目标参与 (TE) 和描述蛋白质与蛋白质相互作用 (PPI) 是药物发现的关键.
由E3酶识别的极性降解子是E3联体设计的有希望的起点,但面临着细胞传递挑战.

研究的目的:

开发有效的在体外和在纤维素中针对基于极性降解蛋白的目标参与 (TE) 策略.
调查聚化细胞透 (CPPs) 的使用,以增强细胞降解序列的传递.
建立一个模型系统,使用含有SPRY域的SOCS盒蛋白2 (SPSB2) E3结合酶.

主要方法:

结合各种多化细胞透 (CPPs) 来降解序列.
高分辨率的晶体结构的确定.
生物物理技术 (例如,SPR,ITC) 用于评估连接物结合和修饰.
基于共聚焦显微镜和生物发光共振能量转移 (BRET) 的测定用于细胞传递和TE确认.

主要成果:

已证明成功地通过细胞传递与CPPs结合的基于degron的.
在纤维素中确认了强烈的目标参与 (TE).
获得了高分辨率的结构洞察力,了解E3联酶-联子相互作用.
使用生物物理方法量化了CPP结合对结合亲和力和细胞吸收的影响.

结论:

聚化CPP可以有效地促进基于极性降解子的的细胞输送.
这种方法使得强大的目标参与 (TE) 成为可能,从而推进了用于PROTACs的E3酶连接体的设计.
这项研究为开发和评估新型E3酶连接体和PROTACs提供了一个强大的平台.

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