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原子力显微镜揭示了Hermetia illucens脂肪酸对MDR细菌的抗菌机制

Heakal Mohamed^1,2, Sergey Leonov³, Elena Marusich⁴

¹Agricultural Research Center (ARC), Plant Protection Research Institute (PPRI), Dokki, Giza, Egypt. heakal2018@gmail.com.

Applied microbiology and biotechnology

|December 8, 2025

概括

来自Hermetia illucens (HI) 幼虫脂肪的脂肪酸对抗多药耐药 (MDR) 病原体具有强大的抗菌活性. 这些天然化合物迅速破坏细菌膜,提供了一个有前途的替代治疗策略.

科学领域:

微生物学微生物学
生物化学生物化学
材料科学材料科学材料科学

背景情况:

多种耐药性 (MDR) 病原体,如 Acinetobacter baumannii 和 Staphylococcus aureus 在医疗保健环境中构成重大威胁.
越来越多的抗菌素耐药性需要开发新的治疗药物.
赫美蒂亚幻影虫 (HI) 幼虫的脂肪是抗菌化合物的潜在来源.

研究的目的:

调查HI幼虫脂肪 (AWME3) 中的脂肪酸 (FA) 对抗MDR A. baumannii和S. aureus的抗菌潜力.
阐明AWME3对这些病原体的作用机制.

主要方法:

脂肪酸是从Hermetia illucens幼虫脂肪 (AWME3) 中提取的.
使用微稀释来评估抗菌活性,以确定最小抑制度 (MIC).
使用酸吸收试验评估了膜透性.
使用原子力显微镜 (AFM) 可视化和量化细胞损伤.

主要成果:

AWME3对A. baumannii (MIC:0.38 mg/mL) 和S. aureus (MIC:0.19 mg/mL) 产生了强大的抑制作用.
在0.75 mg/mL时,在5-10分钟内观察到快速杀菌活性.
AWME3诱导了显著的膜透 (55-70%),ATP耗尽和细胞质泄漏.
AFM揭示了细菌细胞外的纳米损伤,包括孔隙形成和表面崩.

结论:

来自Hermetia illucens幼虫脂肪的脂肪酸对MDR A. baumannii和S. aureus具有显著的抗菌特性.
通过破坏细菌膜的完整性,AWME3迅速起作用.
这些发现凸显了AWME3作为天然抗菌剂对抗医院感染的潜力.

关键词:

赫尔梅蒂亚的幻觉 (英语:Hermetia illucens) 是一种幻觉.航空飞行管理 (AFM)这就是ATPATPATP ATP.脂肪酸是一种脂肪酸.杀人的时间.作用机制作用机制

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