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对法医学体液识别的非向代谢学-试点研究

Meghna Swayambhu¹, Tom D Schneider¹, Janko Tackmann²

¹Zurich Institute of Forensic Medicine, University of Zürich, Zürich 8057, Switzerland.

Analytical chemistry

|December 8, 2025

概括

这项研究引入了一种新的代谢物分析方法,用于识别生物痕迹的体液或组织来源. 这种方法通过提供比当前技术更大的特异性和灵敏性来增强法医调查.

科学领域:

法医科学法医科学法医科学
分析化学分析化学
代谢学代谢学代谢学

背景情况:

目前的体液识别 (BFI) 方法缺乏灵敏度和特异性.
代谢物,作为特定的生物标志物,提供了改善BFI的潜力.
对于法医BFI来说,先进的质谱技术尚未得到充分的探索.

研究的目的:

使用高分辨率质谱测量来研究体液/组织特定的代谢物标记物.
开发一种新的分析方法来识别生物痕迹的起源.
评估代谢学在法医案例工作中的潜力.

主要方法:

采用非定位液体染色学四极飞行时间质谱法 (LC-QTOF-MS).
分析了九种生物液体/组织:便,指针血,月经血液,唾液,精液,皮肤,尿液,阴道液和静脉血液.
使用稀疏的部分最小平方差分分析 (sPLS-DA) 和通用局部学习 (GLL) 来进行特征选择.

主要成果:

确定了负责体液/组织特定聚类的关键代谢物特征.
选择的特征与特定的体液/组织直接相关.
提出了9个预测代谢物特征,每个样本类型都有一个.

结论:

开发的LC-QTOF-MS方法显示了在法医科学中准确识别体液/组织的潜力.
代谢物分析为传统的BFI方法提供了一个有希望的替代方案.
这项试点研究表明,在法医案例工作中使用代谢学是可行的.

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