Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.6K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.6K
RNA Editing02:23

RNA Editing

9.7K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.7K
CRISPR01:59

CRISPR

57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.6K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Broadband 205-GHz vertical-illumination photodiode enabled by double field control layers.

Optics express·2026
Same author

Identification of Urinary Exosomal microRNAs Associated With Subclinical Acute T-Cell-Mediated Rejection in Kidney Transplant Recipients.

Clinical and translational science·2026
Same author

Structural basis of plant organelle C-to-U RNA editing by PPR-DYW proteins.

Nature communications·2026
Same author

Correction: Predictors of lymph node metastases, recurrence, and survival in patients with pedunculated-type T1 colorectal cancer.

Journal of gastroenterology·2026
Same author

Identifying Post-Surgical Recurrence Subtype of T1-Stage Colorectal Cancer by Machine Learning.

Digestion·2026
Same author

Amniotic membrane transplantation promotes retinal hole closure in a rabbit model.

Scientific reports·2025
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
查看所有相关文章

相关实验视频

Updated: Jan 9, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.4K

RECODE:一个可编程的无指导的C-to-URNA编辑工具.

Mizuho Ichinose1, Masaru Ohta1, Yasuka Shimajiri1

  • 1EditForce, Inc., Fukuoka 819-0395, Japan.

Nucleic acids research
|December 8, 2025
PubMed
概括
此摘要是机器生成的。

一个新的无导向RNA编辑工具,RECODE,在人类细胞和小鼠中高效地将cytidine转化为uridine (C-to-U). 这种可编程RNA编辑技术显示了对各种疾病的治疗潜力.

更多相关视频

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.9K
CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

72.7K

相关实验视频

Last Updated: Jan 9, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.4K
A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.9K
CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

72.7K

科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 遗传学 是一个遗传学.

背景情况:

  • 基于CRISPR的RNA细胞因子去胺酶工具提供可编程的C-to-U编辑,但面临低效率和非目标效应等局限性.
  • 现有的RNA编辑系统需要指导RNA,限制可定位序列和灵活性.

研究的目的:

  • 开发一种新的,无指导的RNA编辑工具,用于高效和特定的C-to-U转换.
  • 设计和优化一个工具,以提高编辑活动,减少非目标效果和广泛适用性.

主要方法:

  • 工程RECODE (使用优化DYW酶进行C-to-U的RNA编辑器) 使用RNA结合的三重复蛋白与DYW细胞因子除氨酶域融合.
  • 优化了RECODE的特异性域,用于重定位和减少非目标编辑,并增强了其催化区域,以增加活动和翻译.

主要成果:

  • 在人类细胞中,RECODE在各种目标中展示了高效的C-to-U编辑,在大多数站点实现了50%以上的效率.
  • 该工具具有很高的特异性,避免了对邻近的细胞因子的编辑,并且通过AAV传递在骨肌肉组织中具有高效率的小鼠中被证明是功能性的.

结论:

  • RECODE代表了RNA编辑技术的重大进步,提供了一个无指导,高效和特定的C-to-U转换系统.
  • 证明的活体功能和治疗潜力表明,RECODE可能对治疗各种疾病有价值.