Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Tetraploidy in cancer: Diagnostic and therapeutic perspectives.

Biochemical pharmacology·2026
Same author

ASO Visual Abstract: Immediate Second Look After Cytoreduction for Colorectal or Ovarian Carcinomatosis: An Invisible Gorilla Effect?

Annals of surgical oncology·2026
Same author

Immediate Second Look After Cytoreduction for Colorectal or Ovarian Carcinomatosis: An Invisible Gorilla Effect?

Annals of surgical oncology·2026
Same author

Factor VIII restores bone parameters and modulates muscle proteo-metabolome in Factor VIII knockout male mice.

Bone research·2026
Same author

A Bio-Economic Evaluation of Var, LnVar, and r-Auto Resilience Indicators in Czech Holstein Cattle.

Animals : an open access journal from MDPI·2025
Same author

Microdissection: Insights and progress in the era of precision medicine.

Pathology, research and practice·2025

相关实验视频

Updated: Jan 9, 2026

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
08:30

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

51.9K

从甲固定型胺嵌入的微核样本中比较RNA提取协议.

Maxime Golias1,2,3, Zuzana Krupova1, Pierre Defrenaix1

  • 1Excilone, Elancourt, France.

PloS one
|December 8, 2025
PubMed
概括
此摘要是机器生成的。

分析内异质性至关重要,但目前的方法很昂贵. 一种新的微核技术使用甲固定嵌 (FFPE) 样本显示出对敏感分子应用的希望,即使样本大小小小.

更多相关视频

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

9.7K
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

28.4K

相关实验视频

Last Updated: Jan 9, 2026

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
08:30

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

51.9K
Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

9.7K
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

28.4K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 癌症研究 癌症研究

背景情况:

  • 内异质性分析对于诊断和研究至关重要.
  • 现有的用于组织异质性的剖析技术往往在经济上无法获得,并且在日常使用中不切实际.
  • 嵌入式固定 (FFPE) 样本提供了宝贵的资源,但由于样本大小小和加工,对转录组学研究构成挑战.

研究的目的:

  • 评估五种商业RNA提取套件的有效性,用于分析人类和小鼠FFPE组织中的微核.
  • 使用FFPE微核评估RNA产量,RNA碎片化和RT-qPCR效率.
  • 为了比较从FFPE微核中提取RNA的脱和非脱协议.

主要方法:

  • 从人类 (子宫,胃) 和小鼠 (肝脏,脏) FFPE组织中收集了30个微核样本.
  • 使用了一种新的基于微核的剖析技术,使用200μm针.
  • 在五个商业RNA提取套件中分析了RNA产量,碎片化和RT-qPCR效率,并比较了脱巴拉芬化方法.

主要成果:

  • 在不同套件和FFPE组织类型中观察到RNA产量和碎片化的显著差异.
  • 发现了定量PCR (qPCR) 效率的差异,突出了初始小样本大小的限制.
  • 在脱和非脱的微核样本之间没有发现结果的显著差异.

结论:

  • 证明了使用FFPE微核样本用于敏感分子生物学应用的可行性.
  • 强调了优化工作流程的重要性,因为不同的协议产生了显著不同的结果.
  • 验证了FFPE微核的使用,用于研究组织异质性,有或没有脱化.