从临床样本直接向下一代测序用于诊断耐药结核病 - 一个更新
Radha Gopalaswamy1, Bhargavi Subramanian2, Manisha Parthasarathy3
1ICMR- National Institute for Research in Tuberculosis, Chennai, India.
The Indian journal of tuberculosis
|December 8, 2025
概括
目标下一代测序 (tNGS) 提供了直接从临床样本中快速,全面的结核病 (TB) 耐药性概况. 这种具有成本效益的方法在3-4天内提供了结果,有助于及时管理患者.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 及时检测结核病 (TB) 耐药性对于有效的患者管理至关重要.
- 目前的基因型药物敏感性测试 (DST) 测试在变异覆盖范围和速度方面存在局限性.
- 现型药物敏感性测试 (pDST) 方法是耗时的.
研究的目的:
- 评估目标下一代测序 (tNGS) 作为结核病耐药性概况的快速和全面方法.
- 评估tNGS对直接从临床标本中检测现有和新型结核病药物耐药性的有用性.
主要方法:
- tNGS直接在临床样本上进行,绕过了培养的需要.
- 用商业套件或内部原料增强了DNA目标.
- 测序是在包括Illumina,牛津纳米孔技术和Ion Torrent在内的平台上进行的,利用各种生物信息学管道.
主要成果:
- tNGS提供了全面的药物耐药性概况,从临床样本获得的周转时间为3-4天.
- 该方法可以快速检测对利法素和新型/转型结核病药物的耐药性,如贝达奎林,德拉曼尼德,普雷托曼尼德,莱因佐利德和克洛法齐明.
- 不同的临床样本和DNA提取方法与tNGS兼容.
结论:
- tNGS是一个快速,经济有效和全面的工具,用于直接从临床样本中检测结核病耐药性.
- 与传统方法相比,它具有显著的优势,特别是在识别对较新的结核病药物耐药性的情况下.
- 需要进一步评估,将tNGS整合到结核病诊断算法和临床管理策略中.
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