相关实验视频

Updated: Jan 9, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

使用第三代测序对牙科电梯表面微生物组进行分析:对牙科实践中的感染控制的影响

Jiajia Zheng¹, Kan Wang², Jinghua He¹

¹Eighth Clinical Division, (Former Employer First Clinical Division/Department of Oral and Maxillofacial Surgery), Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, Shijingshan, China.

MicrobiologyOpen

|December 9, 2025

概括

牙科电梯含有多种微生物生物膜,在医疗机构中构成交叉污染风险. 加强绝育协议对于患者安全和感染控制至关重要.

科学领域:

微生物学微生物学
感染控制感染控制
牙科保健牙科保健牙科保健

背景情况:

临床上使用的牙科电梯可以容纳微生物生物膜.
这些生物膜对交叉污染和医院感染有潜在的风险.
牙科环境中的皮革损伤可以促进病原体的传播.

研究的目的:

在牙科升降机上的生物膜中描述微生物群落.
评估交叉污染和医院感染的风险.
评估当前绝育方案的有效性.

主要方法:

在3周内,从15个牙科电梯收集了生物膜样本.
通过PacBio Sequel II平台提取和测序了总DNA.
在测序数据上进行了分类学注释和多样性分析.

主要成果:

鉴定出了多种不同的微生物群落,其中占主导地位的是Prevotella,Fusobacterium,Streptococcus和Lactobacillus.
观察到微生物组成的显著变化和显著的共同殖民模式.
来自Candidatus Saccharibacteria (TM7) 组的未经分类的种群也存在.

结论:

牙科升降机含有复杂的微生物生物膜,增加感染风险.
生物膜形成的异质性需要强有力的感染控制措施.
第三代测序对于理解和改进牙科感染控制策略是有价值的.

关键词:

16S rRNA 基因是16S 的基因.牙科升降机牙科升降机微生物组是一个微生物组.职业暴露职业暴露职业暴露

更多相关视频

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Published on: October 20, 2011

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Published on: December 1, 2014

相关概念视频

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...