纳西克拉辛诱导卡斯巴酶级联激活和细胞亡通过JNK信号在口腔癌
Chun-Wen Su1,2, Shih-Chi Su3,4, Yi-Tzu Chen5,6
1Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
The American journal of Chinese medicine
|December 9, 2025
概括
纳西克拉辛 (NCS) 通过阻止细胞循环进展并诱导口腔状细胞癌 (OSCC) 细胞的亡,有效地对抗口腔癌. 这种天然化合物通过JNK信号激活酶级联,为口腔瘤发生提供治疗潜力.
科学领域:
- 药理学 药理学是指药理学的学科.
- 在瘤学瘤学.
- 自然产品 化学 化学
背景情况:
- 纳西克拉辛 (NCS) 是一种来自Narcissus物种的植物化物,具有已知的抗炎和抗癌特性.
- 关于NCS对口腔状细胞癌 (OSCC) 的具体影响尚未得到广泛研究.
研究的目的:
- 为了研究纳西克拉辛对口腔状细胞癌 (OSCC) 进展的抑制作用.
- 阐明NCS在口腔癌细胞中的作用背后的分子机制.
主要方法:
- 在OSCC细胞系上进行细胞毒性测定.
- 细胞周期分析和细胞亡检测.
- 西方涂抹以评估蛋白质表达 (例如,亡抑制剂) 和酶激活.
- 使用选择性JNK抗剂评估了JNK信号通路的参与.
主要成果:
- NCS对OSCC细胞系表现出显著的细胞毒性.
- 在OSCC细胞中,NCS诱导细胞循环停止并促进细胞亡.
- 通过NCS治疗,可降低亡抑制剂 (HO-2,cIAP-1,survivin,claspin) 的调节,并激活酶级联.
- 发现JNK信号通路对于OSCC中NCS诱导的亡和酶激活至关重要.
结论:
- 纳西克拉辛通过诱导细胞循环停止和细胞亡,有效地抑制OSCC的进展.
- 该机制涉及JNK依赖的酶级联激活和亡抑制剂的下调.
- NCS 呈现了一种有前途的天然治疗剂,用于对抗口腔瘤发生.
相关概念视频
01:31The Intrinsic Apoptotic Pathway
8.2K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.2K
01:24Caspases
13.6K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
13.6K
01:17The Extrinsic Apoptotic Pathway
8.0K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
8.0K
01:07MAPK Signaling Cascades
7.8K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.8K
01:30Apoptosis
13.9K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
13.9K
02:26NF-κB-dependent Signaling Pathway
9.7K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
9.7K