相关实验视频

Updated: Jan 9, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

在腹腔透析患者中,LC-MS/MS 检测出高水平的伊米达 propionate 和来自肠道的代谢物变化

Weerawan Manokasemsan^1,2,3,4, Narumol Jariyasopit^2,3,4, Kwanjeera Wanichthanarak^2,3

¹Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Computational and structural biotechnology journal

|December 9, 2025

概括

一种新的LC-MS/MS方法量化了16种尿素毒素 (UT) 和14种胆酸 (BA). 血分析显示,腹腔透析 (PD) 患者的尿道位升高,突出显示了肠道代谢物对功能衰竭的影响.

科学领域:

代谢学代谢学代谢学
分析化学分析化学
临床化学临床化学

背景情况:

尿素毒素 (UTs) 和胆汁酸 (BAs) 是慢性病 (CKD) 的关键生物标志物.
准确量化这些代谢物对于了解疾病进展和患者的结果至关重要.

研究的目的:

开发和验证一种单一的LC-MS/MS方法,用于同时量化血和便中的16个UT和14个BA.
与健康对照组 (HC) 相比,研究腹透析 (PD) 患者的代谢物概况.

主要方法:

开发一种强大的液体色谱-并联质谱法 (LC-MS/MS) 方法.
在血和便样本中同时定量16个UT和14个BA.
对PD患者 (n=31) 和HC患者 (n=60) 的样本进行分析.

主要成果:

该方法表现出高灵敏度,准确度,精度和吞吐量.
在便中检测到20种代谢物,12种在血中检测到.
血分析显示,PD患者的九种代谢物显著升高,包括硫酸,硫酸和胺 propionate (ImP).
血ImP升高独立于糖尿病或心血管疾病,表明脏清除受损.

结论:

开发的LC-MS/MS平台能够进行全面的UT和BA分析.
在PD患者中血尿路的升高强调了肠道衍生代谢物在功能衰竭中的全身影响.
有针对性的UT-BA分析是CKD和PD临床代谢学的一个有价值的工具.

关键词:

由肠道微生物群衍生的代谢物.功能衰竭功能衰竭是什么?这是LC-MS/MS.腹膜透析是指腹膜透析.量化代谢学量化代谢学量化代谢学尿源性毒素的毒素.

更多相关视频

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

相关概念视频

Dialysis

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...

Peritoneal Dialysis I: Introduction and Procedure

Peritoneal Dialysis I: Introduction and Procedure

Peritoneal dialysis (PD) is a procedure that facilitates the exchange of solutes, waste products, electrolytes, and excess fluid between the blood in the peritoneal capillaries and a dialysis solution introduced into the peritoneal cavity.Principles of Peritoneal Dialysis (PD)Diffusion: Waste products such as urea and electrolytes move from high concentrations in the blood to low concentrations in the dialysate across the peritoneal membrane. This mechanism is driven by the concentration...