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通过调节SLBAM3稳定性,SlCV会影响粉的新陈代谢,SlCV会在番茄的夜间低温压力下调节SlBAM3的稳定性

Jiazhi Lu^1,2,3, Yu Chen^1,2,4, Tianyi Zhang^1,2,5

¹The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China.

Horticulture research

|December 9, 2025

概括

夜间低温的压力导致西红叶中的粉积累. 叶绿体囊泡 (CV) 蛋白通过与β-氨酶3 (BAM3) 相互作用,调节粉分解,影响植物的寒冷耐受性.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生化学

背景情况:

夜间的粉再动员对于植物的碳分配和应激适应至关重要.
β-氨酶3 (BAM3) 是夜间粉降解的主要酶,但其与压力相关的调节尚不清楚.
叶绿体囊泡 (CV) 蛋白在压力下维持叶绿体平衡,但其在粉代谢中的作用尚不清楚.

研究的目的:

为了研究质囊泡 (CV) 蛋白在低夜间温度 (LNT) 压力下调节粉代谢中的作用.
为了阐明SlCV和SlBAM3在寒冷压力下的番茄植物中的相互作用.
了解SlCV介导的SlBAM3调节对植物对LNT压力的耐受性的贡献.

主要方法:

低夜间温度 (LNT) 对番茄植物的应激应用.
对SlCV和SlBAM3表达的基因操纵 (过度表达,沉默,淘汰).
RNA测序 (RNA-seq),代谢组分析和蛋白质与蛋白质相互作用测试.

主要成果:

过度表达SlCV加剧了LNT诱导的粉积累和降低了耐寒性,而SlCV沉默促进了粉的代谢.
SlCV 与 SlBAM3 物理相互作用,而 SlCV 的过度表达在 LNT 压力下加快了 SlBAM3 的降解.
过度表达SlBAM3增强了耐寒性,而SlBAM3补充可以挽救SlCV过度表达植物的LNT过敏性.

结论:

在LNT压力下,SlCV通过促进SlBAM3降解来负面调节粉重新调动.
SlCV-SlBAM3相互作用是控制粉代谢和番茄寒冷耐受性的关键调节机制.
这项研究通过调节粉代谢途径,为植物适应寒冷压力提供了新的见解.

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