血清奥米克分析揭示了洞腔肺结核病中的组织损伤和代谢重编程
Jianhai Wang1,2,3, Dong Zhang4, Chunnan Du1
1Department of Tuberculosis, Haihe Clinical School, Tianjin Medical University, Tianjin 300350, China.
Journal of proteome research
|December 9, 2025
概括
洞腔肺结核 (PTB) 涉及破坏性肺损伤. 研究人员确定了受抑制的细胞结节蛋白和改变的新陈代谢,DSG4和氨基酸盐显示出这种严重的结核病形式的潜在生物标志物.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 腔腔肺结核病 (cPTB) 是一种严重的Mycobacterium结核病感染形式.
- cPTB与治疗结果不佳和高传染性有关.
- 了解cPTB的系统变化对于改善患者护理至关重要.
研究的目的:
- 为了识别与腔腔肺结核相关的系统分子特征.
- 为了比较cPTB,非腔性PTB和健康个体的分子概况.
- 发现cPTB诊断和了解疾病机制的潜在生物标志物.
主要方法:
- 使用近距离延伸蛋白质组学的血清分析.
- 非定位超高性能液态染色学双重质谱法 (UPLC-MS/MS).
- 蛋白质组和代谢组数据的多组分析和综合网络映射.
主要成果:
- 肺结核 (PTB) 显示抑制了细胞结合蛋白和改变了氨基酸/脂质代谢.
- 与非腔性PTB相比,cPTB表现出更明显的代谢变化和心肌细胞相关蛋白质的差异.
- 观察到德斯莫格林-4 (DSG4) 和氨基酸盐的下调,它们的综合水平区分cPTB (AUC = 0.738).
结论:
- 在PTB中形成空洞与心血管路径扰乱和显著的代谢重编程有关.
- 这对DSG4-基酸盐对是cPTB的潜在探索性生物标志物签名.
- 在临床应用或治疗用途之前,需要在独立队列中进一步验证.
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