相关实验视频

Updated: Jan 9, 2026

Rodent Estrous Cycle Monitoring Utilizing Vaginal Lavage: No Such Thing As a Normal Cycle

Rodent Estrous Cycle Monitoring Utilizing Vaginal Lavage: No Such Thing As a Normal Cycle

Published on: August 30, 2021

在雌性周期期间的氧化应激动态:在大鼠模型中的组织学和生物化学发现

Hediye İpek Portakal¹, Ülker Eren², Murat Boyacıoğlu³

¹Department of Histology and Embryology, Institute of Health Sciences, Aydın Adnan Menderes University, Aydın, Turkey.

Histochemistry and cell biology

|December 9, 2025

概括

氧化应激会影响整个卵巢周期的卵巢功能,影响卵泡发育和黄体 (CL) 衰退. 这项研究揭示了氧化应激标志物和抗氧化防御的动态变化,这对生殖健康至关重要.

科学领域:

生殖生物学生殖生物学
细胞生理学细胞生理学
生物化学生化学

背景情况:

氧化应激与卵巢功能和病理学有关.
在雌性周期期间卵巢氧化应激的动态变化尚未得到充分理解.

研究的目的:

为了研究大鼠卵巢组织在不同雌性周期阶段的氧化应激.
为了将氧化应激标志物与毛囊和质动态相关联.

主要方法:

通过阴道涂抹,通过阴道周期分期.
卵巢组织的组织学分析,包括卵泡和红色体 (CL) 计数.
针对8-基-2'-脱氧氨酸 (8-OHdG) 的免疫组合化学染色和针对亡的TUNEL测定.
血清甲酸 (MDA) 和超氧化物脱酶 (SOD) 活性的生物化学分析.

主要成果:

在毛囊细胞,花质细胞和CL细胞中观察到显著的8-OHdG阳性,表明DNA受损.
在雌激 (排卵阶段) 期间血清MDA升高;在雌激期间SOD活动达到峰值.
在CL回归过程中增加的8-OHdG表明氧化应激会影响卵泡发育和白细胞功能.

结论:

氧化应激在整个大鼠雌激循环中起着动态和关键的作用.
研究结果表明氧化应激会影响核和线粒体DNA,影响卵巢细胞命运.
结果对生育调节和生殖医学有意义,突出显示了亡和其他细胞死亡途径的潜在作用.

关键词:

这是一种8-OHdG.气周期的气周期是什么在MDA中,MDA是MDA.氧化应激是一种氧化应激.鼠标鼠标鼠标鼠标这就是SOD的意义.

更多相关视频

Protocol for Studying Extinction of Conditioned Fear in Naturally Cycling Female Rats

Protocol for Studying Extinction of Conditioned Fear in Naturally Cycling Female Rats

Published on: February 23, 2015

Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification

Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification

Published on: September 15, 2012

相关概念视频

Hormonal Control of the Ovarian Cycle

Hormonal Control of the Ovarian Cycle

The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...