从促炎转变为调节性:L. reuteri后生菌在LPS诱导的HT-29IBD模型中调节IL-17/IL-10轴
Ava Behrouzi1, Asal Katebi2, Farhad Riazi Rad3
1Department of Microbiology, TeMS.C., Islamic Azad University, Tehran, Iran.
Archives of microbiology
|December 9, 2025
概括
来自Lactobacillus reuteri的后生物增强了肠道上皮细胞的活力,并减少了IL-17和IL-8等炎症标志物. 这些发现表明,后生菌是治疗炎症性肠道疾病 (IBD) 的有希望的,安全的候选者.
科学领域:
- 胃肠道学和免疫学
- 微生物学和分子生物学
- 细胞生物学和药物发现
背景情况:
- 炎症性肠道疾病 (IBD) 的特征是慢性粘膜炎症和表皮屏障功能受损,通常与失调的细胞因子信号传递有关.
- 目前的治疗方法,如环素有局限性,推动研究更安全的替代品,如益生菌及其副产品,称为后生菌.
研究的目的:
- 在一个体外模型中研究由脂多糖 (LPS) 诱导的HT-29上皮细胞炎症的Lactobacillus reuteri (ATCC 23272) 衍生代谢物的治疗潜力.
- 为了比较这些后生物质与循环素的疗效,这是IBD的常规疗法.
主要方法:
- HT-29细胞受到LPS诱导的炎症,以模仿IBD条件.
- 通过MTT测定来评估细胞活力,并通过Annexin V-FITC流细胞计测量细胞灭亡.
- 细胞因子表达 (IL-4,IL-10,IL-17) 和基因转录 (IL-8,IL-10) 在与L. reuteri后生物和环素治疗后的不同时间点进行了评估.
主要成果:
- 50%的后生物溶液显著增加HT-29细胞活力 (≈140%) 没有表现出细胞毒性.
- 用L. reuteri代谢物治疗导致IL-17+细胞的大量减少 (约为50%) 和IL-10+细胞的轻微增加.
- 到第5天,IL-8基因表达显著下调,效果超过了环素的效果.
结论:
- 乳杆菌reuteri代谢物表现出有利的免疫调节作用,减少细胞亡和关键炎症标志物 (IL-8,IL-17),同时促进IL-10.
- 后生物效应与环素相当或优于环素,突出显示了其在IBD治疗中的潜在稳定性和安全性.
- 建议在肠道器官和动物模型中进行进一步的验证,以确认这些后生物质对IBD的治疗疗效.
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