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通过表达-SELEXEX来选择基DNA酶用于神曲素感应-SELEX

Binfen Chen¹, Yinghong Lin¹, Huixin Chen²

¹Medical School, College of Materials Science and Engineering, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, Institute of Genomics, Huaqiao University, Xiamen 361021, P. R. China.

Analytical chemistry

|December 9, 2025

概括

表达式-SELEX有效地发现用于检测神素的DNA酶. 这些新型DNAzyme生物传感器为临床诊断提供了一个稳定的,经济高效的替代传统的aptamer.

科学领域:

生物化学生物化学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

亚体,DNA或RNA分子,结合联体,但需要表达平台来进行信号转导.
DNA 体提供稳定性和成本效益,但面临着类似的转导挑战.
表达-SELEX将DNA酶表达平台集成到直接信号生成的选择中.

研究的目的:

探索Expression-SELEX的广泛适用性,用于发现各种依赖连接体的DNA酶.
为了识别可响应西奥菲林的全osteric DNAzymes 作为RNA 体的替代品.
使用这些DNAzymes开发功能性生物传感器进行临床检测.

主要方法:

应用Expression-SELEX来分离对神素有反应的DNA酶.
选了12880个独特的候选人,以检测依赖带的裂解活动.
集成的光灭器对用于增强的液相生物传感器测试.

主要成果:

确定了前10名候选人,具有显著的理论素依赖性裂变和对咖啡因的特异性.
在西奥菲林和咖啡因之间进行了有效的歧视.
开发了优化的生物传感器,可在1%-5%的尿液中发挥作用,可通过PAGE凝和液相测试检测.

结论:

表达-SELEX是一种用于快速生成对联体敏感DNA酶的多功能方法.
开发的DNAzyme生物传感器显示出作为具有成本效益的诊断工具的潜力.
这些生物传感器适用于临床检测和生物医学应用.

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Allosteric Regulation

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...