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一个基于ESIPT的光探测器,用于在生物系统中氧化应激过程中可视化pH动态

Qiang Fei¹, Jiayi Yang², Yanbi Luo³

¹State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guiyang, 550014, PR China; Guizhou Engineering Research Center for Characteristic Flavor Perception and Quality Control of Drug-Food Homologous Resources, School of Food Science and Engineering, Guiyang University, Guiyang, 550014, PR China.

|December 9, 2025

概括

研究人员开发了LYB-OH,一种基于本佐醇的光探针,用于监测由氧化应激引起的细胞内pH值波动. 这种探测器对诊断与细胞pH不平衡相关的疾病充满希望.

科学领域:

生物化学生化学
细胞生物学细胞生物学
化学生物学化学生物学

背景情况:

由过度反应性氧物种 (ROS) 标志的细胞氧化应激会破坏平衡,并导致细胞内pH值波动.
细胞内pH值对于细胞平衡,增殖和酶活性至关重要.
监测细胞内pH值对于了解细胞功能障碍至关重要.

研究的目的:

设计和合成一种基于本佐醇的新型光探针LYB-OH,用于有效的细胞内pH值监测.
在体外评估LYB-OH的光物理性质,选择性,稳定性和可逆性.
评估探测器在细胞和斑马鱼pH值变化的生物成像中的性能.

主要方法:

基于佐醇的光探针 LYB-OH.的合成.
光物理性质的表征,包括大型斯托克斯移位 (>200nm在水中).
在体外评估选择性,稳定性和可逆性.
在细胞和斑马鱼中,在细胞和斑马鱼中对氧化应激刺激 (H2O2,LPS) 的反应中pH值变化的细胞内和体内成像.

主要成果:

LYB-OH表现出很大的斯托克斯转移,特别是在水 (>200 nm) 中.
在试验室中,探头表现出良好的选择性,稳定性和可逆性.
在细胞中,LYB-OH 显示出低细胞毒性和有效的pH成像.
探测器成功地可视化了由氧化应激引起的细胞和斑马鱼的细胞内pH值变化.

结论:

LYB-OH是一种有前途的光探针,用于在氧化应激下监测细胞内pH值波动.
探测器可视化pH值变化的能力为诊断与氧化应激和pH值不平衡相关的疾病提供了潜力.
LYB-OH为细胞和体内pH图像应用提供了宝贵的工具.

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