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针对单细胞ICP-MS的特定部位的结合抗体元素标签

Haowen Hu¹, Zhengxian Lv², Yixin Guo¹

¹Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.

|December 9, 2025

概括

我们开发了一种无痕,特定于位点的抗体元素标记 (AET) 策略,用于高度敏感的感应合等离子体质谱 (ICP-MS) 生物分析. 这种方法提高了抗体性能和定量单细胞检测.

科学领域:

生物分子分析.
分析化学是一种分析化学.
纳米技术纳米技术

背景情况:

对于ICP-MS的传统抗体元素标签 (AET) 由于随机修饰而遭受结构异质,影响结合亲和力和特异性.
这种异质性限制了基于ICP-MS的生物分子检测的分析性能和灵敏度.
需要统一和稳定的AET来改善定量生物分析.

研究的目的:

开发一种无痕迹,特定于位点的结合策略,用于创建统一的抗体元素标签 (AET).
提高AETs对ICP-MS的结合亲和力,特异性和分析性能.
为了实现高度敏感和定量单细胞生物分析.

主要方法:

开发了一种无痕,特定于位点的结合策略,将单链DNA (ssDNA) 连接到IgG抗体的K248残留物.
利用ssDNA作为与载有欧 (Eu) 的MS2体纳米粒子混合的链接器,作为信号放大器.
通过将精确的抗体修饰与高容量的MS2元素载体集成,创建了特定站点的AET (ssAET).

主要成果:

开发的ssAETs表现出异常的结构统一性,稳定性和灵敏性.
使用人类表皮生长因子受体2 (HER2) 作为模型,证明了卓越的特异性和定量性能.
与随机结合抗体相比,实现了1.8倍更强的单细胞信号,并标记了1.5倍多的HER2阳性细胞.

结论:

无痕迹,特定站点的ssAET平台为生物分析提供了一种多功能和精确的方法.
这一策略显著提高了使用ICP-MS的单细胞分析的灵敏度和定量性能.
该方法保留了原生抗体结构和结合活性,克服了传统AETs的局限性.

关键词:

抗体元素标签的元素标签在ICP-MS中,使用的是ICP-MS.一个单细胞分析.局部特异性抗体的结合.瘤生物标志物的生物标志物

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