通过高性能液体染色学-单四极质谱学同时识别食补充剂中的50种非法造物质
Takayuki Sakai1, Misa Tanaka1, Yuki Azuma1
1Osaka Institute of Public Health, Division of Hygienic Chemistry, Pharmaceutical Affairs Section, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
Journal of pharmaceutical and biomedical analysis
|December 9, 2025
概括
一种新的液体染色体质谱法 (LC-MS) 方法识别了食补充剂中的50种非法奸剂. 这种先进的技术通过检测未申报的制药成分来提高安全性,保护消费者免受潜在的健康危害.
科学领域:
- 分析化学 分析化学
- 法医科学 法医科学 法医科学
- 药理学 药理学是指药理学的学科.
背景情况:
- 食补充剂可能含有危险的非法奸剂,造成健康风险.
- 现有的伪造物识别方法,如LC-MS,在范围和方便方面都有局限性.
- 由于新型伪造剂的类似物,越来越需要全面的选方法.
研究的目的:
- 开发和验证一种方便的LC-MS方法,用于同时识别在食补充剂中更广泛的非法 adulterants.
- 扩大分析能力,超越先前识别的 adulterants,包括新出现的化合物.
主要方法:
- 开发了一种基于液态色谱-单四极质谱法 (LC-MS) 的方法.
- 该方法被优化为同时识别50种非法造剂,包括新型类似物.
- 方法验证包括线性,特异性 (回收测试),准确性和精度评估.
主要成果:
- 拟议的LC-MS方法实现了所有50个目标非法改剂的清晰分离和识别.
- 观察到优秀的线性 (r2>0.9794,大多>0.99).
- 恢复率在74.9%至127.7%之间,相对标准偏差低于21.3%,这表明32种化合物的特异性和可接受的准确性/精度.
结论:
- 经过验证的LC-MS方法适用于识别50种非法 adulterants,并定量32种在食补充剂.
- 这种方法为检测未申报的制药成分提供了更方便和更全面的方法.
- 该技术在现实世界样本中成功识别了杂质,突出了其在消费者保护方面的实用实用性.
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