与卵巢小白毛囊招募相关的转录组形状的年龄相关变化在商业产卵母的卵巢小白毛囊招募
Suman Poudel1, Vedbar S Khadka2, Rajesh Jha1
1Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI, United States.
Poultry science
|December 9, 2025
概括
由于影响卵巢毛囊招募的分子变化,老年的卵产量减少. 线粒体功能障碍和改变的激素信号通路有助于老家禽的生殖效率下降.
科学领域:
- 禽畜科学 禽畜科学 禽畜科学
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
背景情况:
- 禽类的蛋生产效率与卵巢卵泡动力学有关.
- 生殖效率随着母年龄的增长而下降,但潜在的分子机制尚不清楚.
- 小小的白色毛囊对于排卵和产卵至关重要.
研究的目的:
- 为了确定基因和生物途径,调节老年中小白色卵泡的招募.
- 为了比较不同产卵阶段 (峰值,中期,晚期) 的分子变化.
- 了解老家禽生殖效率下降的基础.
主要方法:
- 从37,67和87周年龄的Hy-line W-36母的小白色毛囊的转录组分析 (RNA测序).
- 使用严格的统计标准识别差异表达基因 (DEGs) (log2折叠变化≥2或≤-2,p值<0.05).
- 定量实时PCR (qPCR) 用于验证.
主要成果:
- 在老中发现了大量的DEG (118个在67周,180个在87周).
- 观察到与线粒体功能,细胞分裂,内细胞分裂,Wnt/β-catenin信号传递,自和mTOR信号传递相关的关键下调基因.
- 包括雌激素,上腺和血清素受体信号在内的抑制途径在老母中被确定,与线粒体功能障碍相伴.
结论:
- 线粒体功能障碍显著影响老年的卵巢卵泡招募.
- 改变的激素信号通路 (雌激素,上腺素,血清素) 导致生殖能力下降.
- 细胞亡和受损的细胞过程是禽类卵巢卵泡发育与年龄相关的下降的关键因素.
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