人类原III片段的重组表达和功能特征
Shuyao Yu1, Kun Wang2, Kangjie Xu2
1Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China; Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China; School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China.
这项研究开发了微生物衍生的原III片段,用于稳定,功能性的皮肤再生生物材料. 优化的复合水凝显著增强纤维细胞活性,用于伤口愈合应用.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 分子生物学分子生物学
背景情况:
- 原III (COL3) 对皮肤结构和修复至关重要.
- 全长度的原体会给稳定性和表达带来挑战.
- 微生物生产为稳定的原蛋白产品提供了一条途径.
研究的目的:
- 使用微生物表达来开发稳定,功能性的原蛋白产品.
- 为了评估原III片段模仿全长蛋白活性.
- 创建用于皮肤再生和伤口愈合的增强生物材料.
主要方法:
- 从人类的 COL3 中提取了功能碎片 (30-60 氨基酸).
- 根据融化温度和净电荷选择了十个碎片.
- 在大肠杆菌中表达的碎片,达到高位数 (高达285 mg/L).
- 将碎片组装成三环结构和纳米纤维.
- 使用样本-308配备的复合水凝,配备了奇托,酸和酸.
主要成果:
- 成功表达了两个可溶性原片段.
- 碎片自组装成三环螺旋结构和纳米纤维.
- 复合水凝,特别是样本-308/SH,显示机械强度增加了10倍.
- 样本-308/SH水凝显著增强纤维细胞 (L929) 粘附,迁移和分化.
- 性能超过了单片段水凝和商业原I.
结论:
- 微生物表达产生了生物活性的COL3碎片.
- 样本-308/SH复合水凝提供了优越的物理性能.
- 这种增强的水凝显示出用于伤口愈合应用的巨大潜力.
- 开发了一种用于组织再生的新型生物材料溶液.
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