微生物组合和便代谢物的变化与对DSS诱导的大肠炎的不同敏感性有关
Jessica M Till1,2,3, Orion D Brock1,2,3, Elyza A Do4
1Department of Cardiovascular and Metabolic Sciences, Cleveland Clinic Research, Cleveland Clinic, Cleveland, Ohio, USA.
Immunology
|December 9, 2025
概括
在老鼠供应商之间肠道细菌的差异影响了炎症性肠病 (IBD) 的易感性. 特定的肠道代谢物可以预测IBD严重程度,为研究免疫微生物群相互作用提供新的途径.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 肠道微生物组的组成因老鼠供应商而异.
- 这种变异与免疫表型和易患炎症性肠病 (IBD) 的易感性有关.
- 功能障碍的免疫微生物群相互作用是IBD的关键驱动因素.
研究的目的:
- 研究小鼠供应商之间的微生物组差异 (查尔斯·里弗与杰克逊) 如何影响肠道免疫力和结肠炎易感性.
- 为了确定与IBD严重程度相关的特定肠道微生物代谢物.
- 建立一个可操作的模型来研究微生物组对IBD的贡献.
主要方法:
- 来自不同供应商的小鼠肠道免疫表型 (Th17,IgA) 的比较.
- 评估对硫酸 (DSS) 诱导的大肠炎的敏感性.
- 共同住房实验,以评估易感性的传播.
- 使用向质谱的便代谢学.
- 代谢物和结肠炎严重程度之间的相关性分析.
主要成果:
- 与杰克逊小鼠相比,查尔斯河小鼠表现出更高的Th17水平和便IgA,以及对DSS诱导的大肠炎的增强敏感性.
- 共同住房表明,CR小鼠将大肠炎易感性传播给JAX小鼠.
- 在敏感和耐药小鼠之间确定了几种差异丰富的便代谢物.
- 特定的代谢物与大肠炎的严重程度相关.
结论:
- 肠道微生物组的自然变异,受供应商特定因素的影响,驱动着对结肠炎的不同易感性.
- 可以确定肠道微生物代谢物,预测IBD严重程度.
- 这项研究建立了一个有价值的小鼠模型,用于进一步调查微生物组-IBD病原体.
相关概念视频
01:17Irritable Bowel Syndrome I: Introduction
893
Irritable Bowel Syndrome (IBS) is characterized by functional disturbances in the gastrointestinal system, presenting a cluster of symptoms without evident structural or biochemical abnormalities. It primarily affects the large intestine and may cause abdominal pain, bloating, excessive gas, diarrhea, constipation, or both.
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
893
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
617
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
617
01:17Chronic Bowel Disorders: Introduction
734
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
734
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
862
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
862