相关实验视频

Updated: Jan 9, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

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通过重组酶 (Redα/β) 增强的DNA整合-CRISPR-Cas9 (RED-CRISPR) 进行高精度高效的转基因敲进

Wenqing Li1, Senquan Liu2,3, Xiaoyu Fang1

  • 1Center for Reproduction and Genetics, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Nature communications
|December 9, 2025
PubMed
概括

介绍RED-CRISPR,这是一种新的基因组编辑工具,使用重组酶 (Redα/β) 来增强CRISPR-Cas9以高效,精确地插入大型DNA有效载荷. 这一突破克服了基因工程在各种应用中的关键局限性.

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