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细菌蛋白质组的进化适应转化阻碍序列

Keigo Fujiwara^1,2,3, Naoko Tsuji⁴, Karen Sakiyama⁴

¹Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan. kig.fujiwara@nig.ac.jp.

The EMBO journal

|December 9, 2025

概括

细菌逮捕,如RAPP和RGPP,可以进化以阻止蛋白质翻译. 这些图案在蛋白质组中很少见,但起着不同的调节作用,显示出双向进化.

科学领域:

微生物学微生物学
分子生物学分子生物学
遗传学遗传学是一种遗传学.

背景情况:

微生物翻译逮捕通过监控细胞环境来调节基因表达.
之前的研究已经确定了具有C端RAPP类序列的细菌逮捕,这些序列位于蛋白质定位基因的上游.

研究的目的:

研究RAPP类序列在细菌转化中的进化动态和调控作用.
为了确定特定的RAPP和RGPP序列,这些序列很容易演变为强大的EF-P耐火转化阻碍序列.

主要方法:

在细菌蛋白质组中对RAPP类序列的比较分析.
在Streptomyces lividans.中识别和描述含有RAPP/RGPP的逮捕.
评估翻译停止效率和EF-P电阻.

主要成果:

RAPP (Arg-Ala-Pro-Pro) 和RGPP (Arg-Gly-Pro-Pro) 序列显示出强烈的倾向,进化为强大的翻译停止,耐受EF-P.
细菌蛋白质组中一般不存在RAPP类图案,这表明转化存在风险.
这些图案通常在小型分泌/膜蛋白的C端附近和具有多种功能的基因上游发现.

结论:

RAPP/RGPP序列表现出双向的进化趋势:从蛋白质体中排除和作为调控性逮捕的适应.
这些,在Streptomyces lividans中发现的各种基因上游,突出了蛋白质定位之外的多种调节作用.
这项研究揭示了细菌中蛋白序列演变,翻译调节和基因功能之间的复杂相互作用.

关键词:

逮捕是一种逮捕.核糖体核糖体核糖体核糖体在SecM SecM 中.翻译逮捕逮捕

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