超声波高压联合处理对大豆蛋白分离物的结构和物理化学特性的影响
Jinying Li1,2, Jiannan Yan3,4, Fangxiao Xing1,2
1College of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin, China.
NPJ science of food
|December 9, 2025
概括
结合超声波和高压处理显著改变了大豆蛋白分离物 (SPI) 的结构,提高了其可溶性,乳化和凝特性,以改善食品应用.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物物理化学 生物物理化学
背景情况:
- 大豆蛋白分离物 (SPI) 是食品中广泛使用的成分.
- 了解处理如何影响SPI结构和功能对于优化其应用至关重要.
- 新的加工技术可以修改蛋白质特性以提高性能.
研究的目的:
- 调查超声波和高压处理对SPI结构和物理化学性质的影响.
- 通过这种综合方法来确定修改SPI的最佳条件.
- 在食品产品开发中探索改性SPI的潜力.
主要方法:
- 红外光谱分析二次结构变化.
- 光光谱检测三级结构.
- 测量工具包用于量化自由硫基和表面疏水性.
- 用于评估凝性质的纹理配置分析.
- 颗粒大小分析和可溶性测量.
主要成果:
- 在SPI中,联合治疗诱导了结构障碍 (二级) 和松的形状 (三级).
- 超声波之后的高压导致了更大的蛋白质展开,增加了热稳定性和增强的表面电荷.
- 表面疏水性,自由硫基和溶解性显著增加 (分别为1.98x,47.74%,1.42x).
- 乳化和凝特性达到最佳水平,平均颗粒大小减少.
结论:
- 结合超声波和高压处理有效地改变了SPI结构和物理化学特性.
- 修改后的SPI表现出更好的功能特性,包括增强的溶解性,乳化和凝.
- 这种修改后的SPI作为一种有价值的加工原料,用于开发高稳定性乳液和高强度凝.
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